Determination of Spiramycin and Oxytetracycline in binary mixtures by novel spectrophotometric methods with and without regression equation
Abou El Khaira, A., Saleh, H., el henawee, M., Ahmed, N., Hussien, E. (2020). Determination of Spiramycin and Oxytetracycline in binary mixtures by novel spectrophotometric methods with and without regression equation. EKB Journal Management System, 58(1&2), 53-61. doi: 10.21608/bfpc.2019.15054.1045
Afaf Abou El Khaira; Hanaa Saleh; magda el henawee; Neven Ahmed; Emad M. Hussien. "Determination of Spiramycin and Oxytetracycline in binary mixtures by novel spectrophotometric methods with and without regression equation". EKB Journal Management System, 58, 1&2, 2020, 53-61. doi: 10.21608/bfpc.2019.15054.1045
Abou El Khaira, A., Saleh, H., el henawee, M., Ahmed, N., Hussien, E. (2020). 'Determination of Spiramycin and Oxytetracycline in binary mixtures by novel spectrophotometric methods with and without regression equation', EKB Journal Management System, 58(1&2), pp. 53-61. doi: 10.21608/bfpc.2019.15054.1045
Abou El Khaira, A., Saleh, H., el henawee, M., Ahmed, N., Hussien, E. Determination of Spiramycin and Oxytetracycline in binary mixtures by novel spectrophotometric methods with and without regression equation. EKB Journal Management System, 2020; 58(1&2): 53-61. doi: 10.21608/bfpc.2019.15054.1045

Determination of Spiramycin and Oxytetracycline in binary mixtures by novel spectrophotometric methods with and without regression equation

Bulletin of Faculty of Pharmacy, Cairo University
Article 5, Volume 58, 1&2, December 2020, Page 53-61  XML PDF (700.56 K)
Document Type: Original Article
DOI: 10.21608/bfpc.2019.15054.1045
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Authors
Afaf Abou El Khaira1; Hanaa Saleh2; magda el henawee3; Neven Ahmed email 4; Emad M. Hussien4
1Faculty of Pharmacy, Zagazig University, Zagazig, Egypt.
2analytical chemistry, Zagazig university, faculty of pharmacy, Zagazig, Egypt
3analytical chemistry, zagazig university, faculty of pharmacy, Zagazig, Egypt
4analytical chemistry, National Organization of Drug Control and Research (NODCAR), Giza, Egypt.
Abstract
Spiramycin (SPI) and oxytetracycline (OTC) are widely used antibacterial drugs. Thus, a great attention has been focusing on the development of a simple and selective analytical procedure for fast analysis of these compounds in a binary pharmaceutical mixture. Herein, we report four simple and reproducible spectrophotometric methods for determination of SPI and OTC in a binary mixture and in veterinary pharmaceutical formulations. A ratio difference (RD) based on the difference in the amplitude of the ratio spectra and dual wavelength methods were proposed for sensitive and selective determination of SPI in presence of OTC. Furthermore, Constant Value and Concentration Value based spectrophotometric methods were suggested for simultaneous determination of SPI and OTC in a binary mixture. The methods were successfully applied for the determination of SPI and OTC over the concentration range from 4.0 to 36.0 µg mL-1 and from 2.0 to 32.0 µg mL-1, respectively. The proposed methods were validated according to the International Conference on Harmonization (ICH) guidelines. The methods are accurate (100±1%), precise (%RSD ≤1.5) and cost-effective for determination of SPI and OTC in binary mixtures.
Keywords
Concentration value; Constant value; Dual wavelength; Oxytetracycline; Ratio difference; Spectrophotometry; Spiramycin
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