CYTOTOXICITY OF IMIDACLOPRID AND MYCLOBUTANIL PESTICIDES ON THREE CANCER CELL LINES
Mohamed, S., Abdel-Megeed, M., Mohamed, K., Ebeed, N., Hammad, A. (2017). CYTOTOXICITY OF IMIDACLOPRID AND MYCLOBUTANIL PESTICIDES ON THREE CANCER CELL LINES. EKB Journal Management System, 25(2), 331-338. doi: 10.21608/ajs.2017.13607
Shaimaa M.S. Mohamed; M. I. Abdel-Megeed; K. A. Mohamed; Naglaa M. Ebeed; A. M. Hammad. "CYTOTOXICITY OF IMIDACLOPRID AND MYCLOBUTANIL PESTICIDES ON THREE CANCER CELL LINES". EKB Journal Management System, 25, 2, 2017, 331-338. doi: 10.21608/ajs.2017.13607
Mohamed, S., Abdel-Megeed, M., Mohamed, K., Ebeed, N., Hammad, A. (2017). 'CYTOTOXICITY OF IMIDACLOPRID AND MYCLOBUTANIL PESTICIDES ON THREE CANCER CELL LINES', EKB Journal Management System, 25(2), pp. 331-338. doi: 10.21608/ajs.2017.13607
Mohamed, S., Abdel-Megeed, M., Mohamed, K., Ebeed, N., Hammad, A. CYTOTOXICITY OF IMIDACLOPRID AND MYCLOBUTANIL PESTICIDES ON THREE CANCER CELL LINES. EKB Journal Management System, 2017; 25(2): 331-338. doi: 10.21608/ajs.2017.13607

CYTOTOXICITY OF IMIDACLOPRID AND MYCLOBUTANIL PESTICIDES ON THREE CANCER CELL LINES

Arab Universities Journal of Agricultural Sciences
Article 8, Volume 25, Issue 2, September 2017, Page 331-338  XML PDF (762.34 K)
Document Type: Original Article
DOI: 10.21608/ajs.2017.13607
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Authors
Shaimaa M.S. Mohamed* 1; M. I. Abdel-Megeed1; K. A. Mohamed1; Naglaa M. Ebeed2; A. M. Hammad1
1Plant Protection Dept., Faculty of Agric., Ain Shams University, Cairo, Egypt
2Genetics Dept., Faculty of Agric., Ain Shams University, Cairo, Egypt
Abstract
Three cancer cell lines, i.e. HEpG-2 (human liver carcinoma), MCF-7 (human breast adeno-carcinoma), and PC3 (Prostatic Small Cell Carcinoma) were used to determine the cytotoxic effects of the neonicotinoid insecticide (imidacloprid) and conazole fungicide (myclobutanil). Cytotoxicity was measured by neutral red incorporation (NRI) assay. The lowest concentration of the tested pesticides (0.5 μg/ml) was toxic. With the increase of the concentration up to 80 μg/ml, the Department of plant protection, Faculty of Agric., Ain Shams University, shoubra  Elkheima, Cairo, Egypt
Department of Genetics, Faculty of Agric., Ain Shams University, Cairo, Egypt damage degree of the cellular form and size was more serious. The midpoint cytotoxicity value, (NRI50) for imidacloprid and myclobutanil for HEpG-2, MCF-7, and PC3 cancer cell lines were 110.5, 67.7 and 67.6 μg/ml and 38.12, 41 and 27.5 μg/ml, respectively. In general, myclobutanil was very toxic in the three cancer cell lines compared with imidacloprid.
 
Keywords
Myclobutanil; Imidacloprid; cell line; Cytotoxicity; NRI assay
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