Evening Primrose Oil/ Vitamin E Combination Treatment Has Renoprotective Effect On Gentamicin-Induced Nephrotoxicity By Suppressing Renal Tumor Necrosis Factor α (TNF-α) / Nuclear Factor κβ (NF- κβ) Pathway And Inhibiting Renal Oxidative Stress | ||||
| Bulletin of Egyptian Society for Physiological Sciences | ||||
| Article 7, Volume 41, Issue 2, April 2021, Page 231-241 PDF (741.07 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/besps.2020.34565.1065 | ||||
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| Authors | ||||
Dalia M Abd-Elhalim1; Mayada Saad Farrag2; Mona Abd-Elbary Hussain  3
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| 1Physiology department, faculty of Medicine, Suez Canal University | ||||
| 2Department of Pathology, Faculty of Medicine, Port Said University, Port Said, Egypt | ||||
| 3Physiology department, Faculty of Medicine, Port Said University, Port Said, Egypt. | ||||
| Abstract | ||||
| Gentamicin is an effective aminoglycoside antibiotic drug but unfortunately up to 30% of gentamicin-treated patients may develop nephrotoxicity. Suggested mechanisms of gentamicin-induced nephrotoxicity included inflammation and oxidative stress injury. Evening primrose oil (EPO) has anti-inflammatory and antioxidant effects. The aim of current study was to assess the possible renoprotective effect of EPO/vitamin E combination treatment on gentamicin-induced nephrotoxicity in rats. Additionally, to address the responsible mechanism(s) of this effect. Eighteen adult male albino Sprague-Dawley rats were equally and randomly divided into three groups; normal control, gentamicin control and EPO/vitamin E treated groups. Gentamicin control group and EPO/vitamin E treated group received IP gentamicin injections for 5 days (100 mg/kg). EPO/vitamin E treated group received EPO/vitamin E (10 g and 200 IU /kg/day respectively orally). Renal function, oxidative stress and histopathological changes were assessed. Renal tissue expressions of tumor necrosis factor α (TNF α) and nuclear Factor κβ (NF- κβ) were assayed. Significant improvements of kidney function markers and tubular necrosis in EPO/vitamin E treated group were observed. EPO/vitamin E treatment significantly ameliorated the gentamicin-induced increase of renal lipid peroxidation and renal tissue expression of TNF α and NF-κβ. EPO/vitamin E treatment significantly ameliorated the gentamicin-induced decrease of renal glutathione and superoxide dismutase concentrations. Conclusion of current study is EPO/vitamin E combination treatment has renoprotective effect on gentamicin-induced nephrotoxicity by inhibiting renal TNF α /NF-κβ signaling pathway and the oxidative stress. Further researches for addressing the renoprotective effect of EPO treatment only and to determine other possible responsible mechanisms are needed. | ||||
| Keywords | ||||
| Evening primrose oil; nephrotoxicity; oxidative stress; tumor necrosis factor α and nuclear factor κβ | ||||
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