SYNERGISTIC EFFECT OF SOME PLANT EXTRACTS AND ANTIBIOTIC DRUGS AGAINST STAPH. AUREUS ISOLATED FROM PLEURAL FLUID IDENTIFICATION OF THE ACTIVE COMPOUNDS
Hussein, A., Gamal, R., Refaat, A., Abdel-Salam, A., Ramadan, K. (2017). SYNERGISTIC EFFECT OF SOME PLANT EXTRACTS AND ANTIBIOTIC DRUGS AGAINST STAPH. AUREUS ISOLATED FROM PLEURAL FLUID IDENTIFICATION OF THE ACTIVE COMPOUNDS. EKB Journal Management System, 25(2), 387-401. doi: 10.21608/ajs.2017.13621
Asmaa F. Hussein; Rawia F. Gamal; A. A. Refaat; A. F. Abdel-Salam; K. M.A. Ramadan. "SYNERGISTIC EFFECT OF SOME PLANT EXTRACTS AND ANTIBIOTIC DRUGS AGAINST STAPH. AUREUS ISOLATED FROM PLEURAL FLUID IDENTIFICATION OF THE ACTIVE COMPOUNDS". EKB Journal Management System, 25, 2, 2017, 387-401. doi: 10.21608/ajs.2017.13621
Hussein, A., Gamal, R., Refaat, A., Abdel-Salam, A., Ramadan, K. (2017). 'SYNERGISTIC EFFECT OF SOME PLANT EXTRACTS AND ANTIBIOTIC DRUGS AGAINST STAPH. AUREUS ISOLATED FROM PLEURAL FLUID IDENTIFICATION OF THE ACTIVE COMPOUNDS', EKB Journal Management System, 25(2), pp. 387-401. doi: 10.21608/ajs.2017.13621
Hussein, A., Gamal, R., Refaat, A., Abdel-Salam, A., Ramadan, K. SYNERGISTIC EFFECT OF SOME PLANT EXTRACTS AND ANTIBIOTIC DRUGS AGAINST STAPH. AUREUS ISOLATED FROM PLEURAL FLUID IDENTIFICATION OF THE ACTIVE COMPOUNDS. EKB Journal Management System, 2017; 25(2): 387-401. doi: 10.21608/ajs.2017.13621

SYNERGISTIC EFFECT OF SOME PLANT EXTRACTS AND ANTIBIOTIC DRUGS AGAINST STAPH. AUREUS ISOLATED FROM PLEURAL FLUID IDENTIFICATION OF THE ACTIVE COMPOUNDS

Arab Universities Journal of Agricultural Sciences
Article 14, Volume 25, Issue 2, September 2017, Page 387-401  XML PDF (1.04 MB)
Document Type: Original Article
DOI: 10.21608/ajs.2017.13621
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Authors
Asmaa F. Hussein* 1; Rawia F. Gamal1; A. A. Refaat1; A. F. Abdel-Salam2; K. M.A. Ramadan3
1Agricultural Microbiology Dept., Faculty of Agriculture, Ain Shams University, Cairo, Egypt
2Bacterial Contamination Dept., ARC, Giza, Egypt
3Biochemistry Dept., Faculty of Agriculture, Ain Shams University, Cairo, Egypt
Abstract
A total of 75 pyogenic samples were collected from patients examinated for pyogenic infection in Sednawy hospital and Elmokhtaber laboratories. S. aureus isolated from pleural fluid out of five S. aureus (+ve coagulase, MSSA) exhibits resistant against all the concentration of Ciprofloxacin 0.5-20, Gentamycin 0.5- 5.0 µg/100µl and Amikacin 1 µg/100µl. The highest potent of phytoextracts either extracted by water or methanol was detected by clove comparing with mint, thyme, sage and garlic, whereas garlic essential oil gave completely abolish of S. aureus (P.F). The highest synergism was obtained in combination between the lowest inhibitory concentration of Gentamycin 10 µg/100µl  or Vancomycin 1 µg/100µl or Amikacin 2.5 µg/100µl with thyme Eso (1:3), which resulted to increase the efficacy by 4.4 or 1.5 or 1.6 respectively  comparing to recommended dose of the tested antibiotics alone. Increasing the concentration of garlic essential oil (GEso) from 10 up to 100 µl/well increment the efficacy of inhibition up to 10, 3.7 and 4.5- fold when standard dose of Gentamycin, Vancomycin and Amikacin were used individually against S. aureus P.F. The minimum bactericidal concentration of garlic essential oil was recorded at 2 µl/ml as it resulted to reduce the count to be 0.04%. Analysis of garlic essential oil by GC-MS dedicated six sulfur compounds represented 88.8644% of total detected compounds in garlic essential oil.
Keywords
S.aureus; antibiotics; Phytoextract; synergistic effects; MBC; 16S rRNA; GC-MS spectrometry
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