RAPD-evaluated Genetic Polymorphisms and Relations among three River Nile Catfish (Siluriformes) Species from Qena, Egypt | ||||
| Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology | ||||
| Article 2, Volume 10, Issue 1, June 2018, Page 9-21 PDF (610.1 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/eajbsc.2018.13652 | ||||
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| Author | ||||
| Mohammed Bassyouni M. EL-Mahdi | ||||
| Laboratory of Molecular Genetics and Molecular Biology, Zoology Department Faculty of Science, South Valley University, Qena, Egypt | ||||
| Abstract | ||||
| Genetic discrepancies among three River Nilecatfish; Schilbe mystus Linnaeus, 1758 (Schilbeidae), Bagrus bajad Forsskål, 1775 (Bagaridae) and Clarias gariepinus Burchell, 1822 (Claridae) was evaluated by RAPD-PCR technique using 8 deca oligonucleotides (A-03, A-04, A-05, A-06, A-09, A-10, A-11, and A-12). A total of 52 amplified bands were produced, from them 5 bands were common with level of monomorphism of % 9.62, and 47 bandswere polymorphicwith level of polymorphism of 90.38%. An instructive RAPD fingerprint profile was generated with various band size lengths ranging from 200 to 2900 base pair. According to Nei-72 distance matrix of genetic and the unweighted pair group method average (UPGMA), the three studied catfish species are related to each other and likely have the most sharing common ancestor. However, Schilbe mystus (Schilbeidae)and Bagrus bajad (bagaridae) are genetically more close to each other than to Clarias gariepinus. The subset of Clarias gariepinus as sister clade to Schilbe mystus and Bagrus bajad suggested monophyly of the family Clarridae. The study suggested occurrence of genetic variations among the investigated River Nile catfish, with high level of genetic convergence/relation between Schilbe mystus and Bagrus bajad. This probably be useful for enhancing their potential use in aquaculture breeding programs, as well as providing insights on their taxonomic status within the Siluriformes. | ||||
| Keywords | ||||
| genetic polymorphism; catfish; Siluformes; RAPD-PCR; Qena; River Nile; Egypt | ||||
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