Identification of Ganoderma Isolates from Egypt Based on Morphological Characters and ITS1-rDNA Genetic Marker
Reda, L., Ebeed, N., EL-Samman, M., Mostafa, M., Ahmed, M. (2017). Identification of Ganoderma Isolates from Egypt Based on Morphological Characters and ITS1-rDNA Genetic Marker. EKB Journal Management System, 9(2), 21-38. doi: 10.21608/eajbsc.2017.13664
Labiba Ahmed Reda; Naglaa M. Ebeed; M. G.E.M. EL-Samman; M. H. Mostafa; M. A. Ahmed. "Identification of Ganoderma Isolates from Egypt Based on Morphological Characters and ITS1-rDNA Genetic Marker". EKB Journal Management System, 9, 2, 2017, 21-38. doi: 10.21608/eajbsc.2017.13664
Reda, L., Ebeed, N., EL-Samman, M., Mostafa, M., Ahmed, M. (2017). 'Identification of Ganoderma Isolates from Egypt Based on Morphological Characters and ITS1-rDNA Genetic Marker', EKB Journal Management System, 9(2), pp. 21-38. doi: 10.21608/eajbsc.2017.13664
Reda, L., Ebeed, N., EL-Samman, M., Mostafa, M., Ahmed, M. Identification of Ganoderma Isolates from Egypt Based on Morphological Characters and ITS1-rDNA Genetic Marker. EKB Journal Management System, 2017; 9(2): 21-38. doi: 10.21608/eajbsc.2017.13664

Identification of Ganoderma Isolates from Egypt Based on Morphological Characters and ITS1-rDNA Genetic Marker

Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
Article 3, Volume 9, Issue 2, December 2017, Page 21-38  XML PDF (773.41 K)
Document Type: Original Article
DOI: 10.21608/eajbsc.2017.13664
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Authors
Labiba Ahmed Reda1; Naglaa M. Ebeed2; M. G.E.M. EL-Samman3; M. H. Mostafa3; M. A. Ahmed3
1Plant Pathology Dept., Fac. Agri., Ain Shams Unv., Shoubra El- khima, Cairo, Egypt
2Genetics Dept., Fac. Agri., Ain Shams Unv., Shoubra El- khima, Cairo, Egypt
3Plant Pathology Dept., Fac. Agri., Ain Shams Unv., Shoubra El- khima, Cairo, Egypt.
Abstract
The basidiomycete fungus Ganoderma Karst., a polyporoid genus within the family Ganodermataceae of the order Aphyllophorales, is worldwide in distribution. The accurate identification of the Ganoderma is still controversial particularly for the tropical species due to high variability in the basidiocarp morphology, and complicated speciation which leads to misidentification by traditional taxonomic methods.
Specimen of Ganoderma basidiocarps were collected from different hosts (Navel orange, Oil palm, Fan palm, Casuarina and Morus) in Giza and Qalyubia governorates, Egypt and were identified to species level according to its morphological characters  as well as PCR and nucleotide sequence analysis of the ribosomal 5.8S r-DNA gene and the flanking internal transcribed spacers (ITS) utilizing specific primers with ITS 1 region as a target. Isolates of Ganoderma resinaceum were described for the first time in Egypt, where, morphological and cultural observations and phylogenetic analysis of ITS1 sequences revealed that all isolates collected from infected trees belong to a single species Ganoderma resinaceum.
Keywords
Ganoderma resinaceum; Identification Micromorphology rDNA-ITS1 Phylogeny
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