Using PCR for detection of Borrelia sp. in Ticks and Ruminants in Egyptian Desert
Abdel-Baky, S., Allam, N., Kamel, Y., Hafez, A. (2020). Using PCR for detection of Borrelia sp. in Ticks and Ruminants in Egyptian Desert. EKB Journal Management System, 21(4), 167-172. doi: 10.21608/mvmj.2020.136907
Sayed Abdel-Baky; Nesreen Allam; Yasser Kamel; Amani Hafez. "Using PCR for detection of Borrelia sp. in Ticks and Ruminants in Egyptian Desert". EKB Journal Management System, 21, 4, 2020, 167-172. doi: 10.21608/mvmj.2020.136907
Abdel-Baky, S., Allam, N., Kamel, Y., Hafez, A. (2020). 'Using PCR for detection of Borrelia sp. in Ticks and Ruminants in Egyptian Desert', EKB Journal Management System, 21(4), pp. 167-172. doi: 10.21608/mvmj.2020.136907
Abdel-Baky, S., Allam, N., Kamel, Y., Hafez, A. Using PCR for detection of Borrelia sp. in Ticks and Ruminants in Egyptian Desert. EKB Journal Management System, 2020; 21(4): 167-172. doi: 10.21608/mvmj.2020.136907

Using PCR for detection of Borrelia sp. in Ticks and Ruminants in Egyptian Desert

Mansoura Veterinary Medical Journal
Article 5, Volume 21, Issue 4, December 2020, Page 167-172  XML PDF (1.38 MB)
Document Type: Original Articles
DOI: 10.21608/mvmj.2020.136907
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Authors
Sayed Abdel-Baky* 1; Nesreen Allam2; Yasser Kamel3; Amani Hafez3
1Parasitology Unit, Department of Animal Health, Division of Animal and Poultry Production, Desert Research Center, Matariya, Cairo, P.O. Box: 11562, Egypt.
2Parasitology and Animal Diseases Department, Veterinary Research Division, National Research Centre, P.O. Box: 12622, Dokki, Egypt
3Infectious Diseases Unit, Department of Animal Health, Division of Animal and Poultry Production, Desert Research Center, Matariya, Cairo, P.O. Box: 11562, Egypt
Abstract
Objective: To investigate the prevalence ofBorrelia burgdorferi infection in ticks and ruminant hosts.
Design: Cross sectional study.
Animals: 739 Ixodes ticks, 110 camels, 120 sheep and 40 cattle.
Procedures:  All collected samples were tested by using nested PCR technique (nPCR) using OspA and flaB gene as the targets.
Results: Out of 739 adult Ixodes ticks, 111 (15%) were infected, the highest rate was in A. variegatum (43.24%) followed by A. lepidum (30.63%) and B. annulatus (26.13%). Shalatten was the most infected locality (18.52%) followed by Dakhla Oasis and Bir El-Abid in ratio 18.52% and 16.05%, respectively. Camel was the most infected animal (40.74%), then sheep (37.04%) and cattle (22.22%).
Conclusion and Clinical relevance: Medical and veterinary professionals in Egypt should consider B. burgdorferi as an emerging pathogen in the country. Future studies are needed to monitoring vertorial capacity of ticks in transmission B. burgdorferi to animals in parallel with discovering other reservoir host animals and the vector competence of tick species for B. burgdorferi and its public health and economic significance in Egypt.
Keywords
Borrelia burgdorferi; Borreliosis; nPCR; Ixodes ticks
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