Bioinformatic Analysis of the Beauvericin Gene from Beauveria bassiana and Insecticidal Effect on Spodoptera littoralis (Boisd)
Ali, S., El-sayed, Y. (2015). Bioinformatic Analysis of the Beauvericin Gene from Beauveria bassiana and Insecticidal Effect on Spodoptera littoralis (Boisd). EKB Journal Management System, 7(2), 89-99. doi: 10.21608/eajbsc.2015.13694
Sahar S. Ali; Yasmin A. El-sayed. "Bioinformatic Analysis of the Beauvericin Gene from Beauveria bassiana and Insecticidal Effect on Spodoptera littoralis (Boisd)". EKB Journal Management System, 7, 2, 2015, 89-99. doi: 10.21608/eajbsc.2015.13694
Ali, S., El-sayed, Y. (2015). 'Bioinformatic Analysis of the Beauvericin Gene from Beauveria bassiana and Insecticidal Effect on Spodoptera littoralis (Boisd)', EKB Journal Management System, 7(2), pp. 89-99. doi: 10.21608/eajbsc.2015.13694
Ali, S., El-sayed, Y. Bioinformatic Analysis of the Beauvericin Gene from Beauveria bassiana and Insecticidal Effect on Spodoptera littoralis (Boisd). EKB Journal Management System, 2015; 7(2): 89-99. doi: 10.21608/eajbsc.2015.13694

Bioinformatic Analysis of the Beauvericin Gene from Beauveria bassiana and Insecticidal Effect on Spodoptera littoralis (Boisd)

Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
Article 10, Volume 7, Issue 2, December 2015, Page 89-99  XML PDF (714.9 K)
Document Type: Original Article
DOI: 10.21608/eajbsc.2015.13694
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Authors
Sahar S. Ali; Yasmin A. El-sayed
Plant Protection Research Institute, Agricultural Research Center, Dokki, Giza, Egypt
Abstract
Beauvericin, a cyclohexadepsipeptide-possessing natural product with synergistic antifungal, insecticidal, and cytotoxic activitie. Total DNA was extracted from B. bassiana Egyptian isolate. The integrity of total DNA was estimated by ethidium bromide re, taq DNA polymerase and (Forward and Reverse) primers directly which designed to amplify the beauvericin gene. The nucleotide sequence of the PCR-amplified fragment for the beauvericin gene of B. bassiana EG-isolate was done to determine the relationship with other B. bassiana isolates registered in Gen Bank, and was aligned by using DNAMAN program (Wisconsin, Madison, USA) with another three B. bassiana  isolates. The predict numbers of amino acids were produced from translation of beauvericin gene nucleotide sequence were 211 amino acids. A phylogenetic tree of beauvericin from B. bassiana Eg. isolate revealed 100% a high degree of similarity to beauvericin of B. bassiana non ribosomal cyclo depsipeptide synthetase, (Accession no.  AC130655), 94.3% and 94.8% to beauvericin of B. bassiana biosynthetic  protein (Accession no.  ADO 60131) and beauvericin of B. bassiana biosynthetic protein, (Accession no.  AFJ44691) respectively. While, the insecticidal effect of toxins crude extraction was studied in this wake . Toxins crude extraction due to isolate of B. bassiana investigated against 3rd inster larvae of S. littoralis. After 4 days, the percentage of mortality  were 51.00%, 57.50%, 79.00% and 96.50% in the concentrations 25, 50, 75 and 100%, respectively.
Keywords
Bioinformatic; the beauvericin gene; Beauveria bassiana Spodoptera littoralis (Boisd)
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