RAPD-Based Genetic Variances Between Female and Male Adult House Fly Musca domestica L.1758 (Diptera, Muscidae) From Qena, Egypt | ||||
| Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology | ||||
| Article 13, Volume 7, Issue 2, December 2015, Page 127-134 PDF (843.97 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/eajbsc.2015.13697 | ||||
 View on SCiNiTO | ||||
| Author | ||||
| Mohammed Bassyouni M. EL-Mahdi | ||||
| Laboratory of Molecular Genetics and Molecular Biology, Zoology Department Faculty of Science, South Valley University, Qena, Egypt | ||||
| Abstract | ||||
| The genomic DNA from female and male adults house fly Musca domestica L. was subjected to theRAPD analysis using a set of random primers. A ten (10) selected primers produced a distinctive RAPD fingerprint of various bands ranging in size from 350 to 1700 bp. A total of 57 RAPD bands/loci were produced with an average of 5.7 bands per primer, from them 18 bands with a level of monomorphism of 31.58% were common between female and male, and 39 were polymorphic corresponding to a level of polymorphism of 68.42 %. Both female and male genotypes displayed high band frequencies, however male showed to be slightly higher than female ones. Result of this study suggested occurrence of gender-based internal genetic variations and heterozygosity of common house fly adults Musca domestica L. that is slightly higher in males than females. This would be necessitated for the species survival, successful environmental adaptations and resistance to chemical applications. | ||||
| Keywords | ||||
| Genetic variances; RAPD-PCR; genotype; Diptera; Musca domestica; Qena | ||||
|
Statistics Article View: 244 PDF Download: 343 |
||||
