Molecular phylogenetic relationship among three Acanthodactylus species (Reptilia: Lacertidae) in Egypt based on 18S rRNA Sequences
Sarhan, M., Saber, I., El-Shehaby, M., Saber, S. (2015). Molecular phylogenetic relationship among three Acanthodactylus species (Reptilia: Lacertidae) in Egypt based on 18S rRNA Sequences. EKB Journal Management System, 7(1), 39-49. doi: 10.21608/eajbsc.2015.13701
Moustafa Sarhan; Islam Saber; Mohamed El-Shehaby; Samy Saber. "Molecular phylogenetic relationship among three Acanthodactylus species (Reptilia: Lacertidae) in Egypt based on 18S rRNA Sequences". EKB Journal Management System, 7, 1, 2015, 39-49. doi: 10.21608/eajbsc.2015.13701
Sarhan, M., Saber, I., El-Shehaby, M., Saber, S. (2015). 'Molecular phylogenetic relationship among three Acanthodactylus species (Reptilia: Lacertidae) in Egypt based on 18S rRNA Sequences', EKB Journal Management System, 7(1), pp. 39-49. doi: 10.21608/eajbsc.2015.13701
Sarhan, M., Saber, I., El-Shehaby, M., Saber, S. Molecular phylogenetic relationship among three Acanthodactylus species (Reptilia: Lacertidae) in Egypt based on 18S rRNA Sequences. EKB Journal Management System, 2015; 7(1): 39-49. doi: 10.21608/eajbsc.2015.13701

Molecular phylogenetic relationship among three Acanthodactylus species (Reptilia: Lacertidae) in Egypt based on 18S rRNA Sequences

Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
Article 4, Volume 7, Issue 1, September 2015, Page 39-49  XML PDF (577.29 K)
Document Type: Original Article
DOI: 10.21608/eajbsc.2015.13701
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Authors
Moustafa Sarhan1; Islam Saber1; Mohamed El-Shehaby2; Samy Saber3
1Department of Zoology, Faculty of Science, Al-Azhar University, Assiut
2Department of Zoology, Faculty of Science, Al-Azhar University,
3Department of Zoology, Faculty of Science, Al-Azhar University, Cairo
Abstract
Acanthodactylus is a genus of lacertid lizard, commonly referred to as fringe-fingered lizards or fringe-toed lizards. The systematics of the genus Acanthodactylus was classically based on external morphological traits, osteological characters and morphology of the hemipenes. In this study, partial sequences of 18S rRNA gene were used to estimate the relationships among three lacertide species from Egypt: Acanthodactylus boskianus, Acanthodactylus scutellatus and  Acanthodactylus  longipes. These data were used together with morphological information, to evaluate the relationships among these three species. DNA was extracted from the collected specimens, 988 bp fragment of 18S rRNA was amplified using universal primers and sequenced. The obtained sequences were analyzed and a phylogenetic tree was constructed. Results of analysis revealed that A. scutellatus and A. longipes form a very well supported clade with 86% bootstrap. A. boskianus was found to constitute a separate clade.  The resultant tree based on DNA sequence data was similar to that produced from a differentially weighted data set of morphological characters. In conclusion, the 18S rRNA gene sequence seems to be a good marker for phylogenetic analysis of Acanthodactylus species.
Keywords
Lacertidae; Acanthodactylus 18S rRNA Phylogeny Sequencing
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