Biological and molecular aspects of the insect growth regulator lufenuron on Spodoptera littoralis (Bosid) (Lepidoptera:Noctuidae)
Bakr, R., El-barky, N., Abd Elaziz, M., Awad, M., Abd El-Halim, H. (2014). Biological and molecular aspects of the insect growth regulator lufenuron on Spodoptera littoralis (Bosid) (Lepidoptera:Noctuidae). EKB Journal Management System, 6(2), 1-16. doi: 10.21608/eajbsc.2014.13709
Reda F. A. Bakr; Nehad M. El-barky; Mona F. Abd Elaziz; Mohamed H. Awad; Hisham M. E. Abd El-Halim. "Biological and molecular aspects of the insect growth regulator lufenuron on Spodoptera littoralis (Bosid) (Lepidoptera:Noctuidae)". EKB Journal Management System, 6, 2, 2014, 1-16. doi: 10.21608/eajbsc.2014.13709
Bakr, R., El-barky, N., Abd Elaziz, M., Awad, M., Abd El-Halim, H. (2014). 'Biological and molecular aspects of the insect growth regulator lufenuron on Spodoptera littoralis (Bosid) (Lepidoptera:Noctuidae)', EKB Journal Management System, 6(2), pp. 1-16. doi: 10.21608/eajbsc.2014.13709
Bakr, R., El-barky, N., Abd Elaziz, M., Awad, M., Abd El-Halim, H. Biological and molecular aspects of the insect growth regulator lufenuron on Spodoptera littoralis (Bosid) (Lepidoptera:Noctuidae). EKB Journal Management System, 2014; 6(2): 1-16. doi: 10.21608/eajbsc.2014.13709

Biological and molecular aspects of the insect growth regulator lufenuron on Spodoptera littoralis (Bosid) (Lepidoptera:Noctuidae)

Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
Article 1, Volume 6, Issue 2, December 2014, Page 1-16  XML PDF (975.78 K)
Document Type: Original Article
DOI: 10.21608/eajbsc.2014.13709
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Authors
Reda F. A. Bakr1; Nehad M. El-barky2; Mona F. Abd Elaziz2; Mohamed H. Awad3; Hisham M. E. Abd El-Halim2
1-Entomology Department – Faculty of Science- Ain Shams University -Biology Department –Faculty of Science –KKU- Abha-KSA
2Entomology Department – Faculty of Science- Benha University
3Zoology Department – Faculty of Science- Benha University
Abstract
The cotton leafworm, S. littoralis, isa major pest in Egypt causes severe quantitative and qualitative losses of cotton and other economic crops. This study examined the effect of chitin synthesis inhibitors, lufenuron (match 5%) on the 2nd instar larvae. The results showed that lufenuron caused not only mortality in larval stages, but also caused effect on pupal and adult duration, adult fecundity and egg fertility. Also lufenuron treatments have resulted in emergence of deformed pupae and adults. PCR-RFLP technique was used in this study to investigate the effect of lufenuron on the vira-like chitinase gene, one of the genes involved in the formation of chitin and concerned with development of insect. The endonucleases Spel, EcoRI and Bbsl were used to detect the molecular changes at different time intervals 6, 12, 24 and 48hrs post treatment with sublethal doses LC25, LC50 and LC90.The results showed significant changes in the sequencesof the treated groups which weredose and time dependants. Moreover the number of RNA secondary structure stems was varied in the different studied groups and most of them differed in their nucleotide sequences, positions and free energy.
Keywords
lufenuron; Spodoptera littoralis; biological; Molecular Aspects
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