Insecticidal Activity and Biochemical Study of the Clove Oil (Syzygium aromaticum) Nano- Formulation on Culex pipiens L. (Diptera: Culicidae) | ||||
| Egyptian Journal of Aquatic Biology and Fisheries | ||||
| Article 12, Volume 25, Issue 1, January and February 2021, Page 227-239 PDF (681.89 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejabf.2021.137233 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| El Gohary E. El Gohary; Shaimaa M. Farag; Ahmed A. El-Sayed; Reham R. Khattab; Dalia M. Mahmoud | ||||
| Abstract | ||||
| In the present study Syzygium aromaticum essential oil (EO) was extracted using water as a solvent and encapsulated within chitosan nanoparticles, which are characterized by Ultraviolet-Visible (UV-VIS) spectra, Infra-Red (IR), Transmission Electron Microscopy (TEM), and X-ray diffraction (XRD). The insecticidal activity for both Syzygium aromaticum bulkEO and encapsulated EO within chitosan nanoparticles was determined. Both of the two oil forms were assessed as a larvicide against third instar larvae of Culex pipiens by measuring LC values. Encapsulated nano-formulation EO showed higher toxicity (LC50 =20 ppm) than the bulk EO (LC50 =39 ppm). The biochemical changes were measured after treatment with both forms of EO. There is an increase in the activity of three enzymes (acid phosphatase, alkaline phosphatase, and glutathione-S-transferase), while the activity of acetylcholinesterase was decreased. The obtained results revealed that water extract of Syzygium aromaticum EO and nano-encapsulated EO may be promising alternative larvicide for controlling Culex pipiens larvae in integrated pest management. | ||||
| Keywords | ||||
| Biochemistry; Culex pipiens; encapsulation; insecticidal; nano-chitosan; Syzygium aromaticum | ||||
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