Clonal Diversity and High Prevalence of Oxa-23 among Carbapenem-Resistant Acinetobacter baumannii Isolates in Egypt
Fam, N., Gamal, D., Salem, D., Dahroug, H., Wasfy, R., Morcos, M. (2019). Clonal Diversity and High Prevalence of Oxa-23 among Carbapenem-Resistant Acinetobacter baumannii Isolates in Egypt. EKB Journal Management System, 5(1), 110-124. doi: 10.21608/jbaar.2019.138331
Nevine Fam; Doaa Gamal; Dalia Salem; Heba Dahroug; Reham M. Wasfy; Myriam M. Morcos. "Clonal Diversity and High Prevalence of Oxa-23 among Carbapenem-Resistant Acinetobacter baumannii Isolates in Egypt". EKB Journal Management System, 5, 1, 2019, 110-124. doi: 10.21608/jbaar.2019.138331
Fam, N., Gamal, D., Salem, D., Dahroug, H., Wasfy, R., Morcos, M. (2019). 'Clonal Diversity and High Prevalence of Oxa-23 among Carbapenem-Resistant Acinetobacter baumannii Isolates in Egypt', EKB Journal Management System, 5(1), pp. 110-124. doi: 10.21608/jbaar.2019.138331
Fam, N., Gamal, D., Salem, D., Dahroug, H., Wasfy, R., Morcos, M. Clonal Diversity and High Prevalence of Oxa-23 among Carbapenem-Resistant Acinetobacter baumannii Isolates in Egypt. EKB Journal Management System, 2019; 5(1): 110-124. doi: 10.21608/jbaar.2019.138331

Clonal Diversity and High Prevalence of Oxa-23 among Carbapenem-Resistant Acinetobacter baumannii Isolates in Egypt

Journal of Bioscience and Applied Research
Article 10, Volume 5, Issue 1, March 2019, Page 110-124  XML PDF (734.38 K)
Document Type: Original Article
DOI: 10.21608/jbaar.2019.138331
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Authors
Nevine Fam1; Doaa Gamal1; Dalia Salem email 1; Heba Dahroug1; Reham M. Wasfy1; Myriam M. Morcos2
1Department of Microbiology, Theodor Bilharz Research Institute (TBRI), Giza, Egypt
2Naval Medical Research Laboratories (NAMRU-3). Cairo, Egypt
Abstract
Background and Aim: Carbapenem-resistant Acinetobacter baumannii (CRAB) is becoming a global threat especially to hospitalized patients. We aimed to address the magnitude of CRAB causing healthcare-associated infections in patients admitted to a tertiary healthcare hospital in Egypt and to study their genetic and epidemiologic diversity. Materials and Methods: Twenty-six CRAB isolates representing 48% of all Acinetobacter baumannii (A. baumannii) isolated in the study period were identified by microbiological culture methods and verified by the presence of bla-oxa51. Antimicrobial susceptibility was tested by disc diffusion and MIC was determined by VITEK2 compact system. Phenotypic expression of metallo-β-lactamases (MBLs) was determined by MBL IP/IPI E-test. Carbapenemase encoding genes were identified by PCR and clonal relatedness was studied by pulsed-field gel electrophoresis (PFGE) using Apa1 and PulseNet protocol. Results: All A. baumannii isolates were multi-drug resistant (MDR). Colistin and minocycline showed the highest sensitivities of 100% and 61.1%respectively. MBLs were phenotypically detected in 20/26 (76.9%) of the isolates while blaOXA-23-like was the main carbapenem resistance gene recorded in 61.5% followed by blaNDM-1-like (26.9%) and blaGES-like (7.7%).  PFGE typing showed high diversity as most of the isolates were < 80% similar. Conclusion: Carbapenem resistance among A. baumannii isolates is increasing dramatically in our geographic region. BlaOXA-23-likeis the most common gene in CRAB isolates in our hospital setting. Also, blaNDM-1-like and blaGES-like harboring A. baumannii isolates are exhibiting a considerable spread in the hospital environment in Egypt. The clonal diversity of our CRAB isolates suggests that it could be due to the horizontal dissemination of mobile genetic elements rather than the propagation of a certain clone. 
Keywords
A. baumannii; carbapenem resistance; GES; NDM; OXA-23; PFGE
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