Isolation and characterization of MHCIIA gene from Nile tilapi a | ||||
Delta Journal of Science | ||||
Article 9, Volume 40, Issue 1, June 2019, Page 78-87 PDF (1.54 MB) | ||||
Document Type: Research and Reference | ||||
DOI: 10.21608/djs.2019.139211 | ||||
View on SCiNiTO | ||||
Authors | ||||
Aml A. El-Semlawy1; Karim S. El-Said1; Mohammed A. El-Magd2; Tarek M. Mohamed* 1 | ||||
1Department of Chemistry (Biochemistry section), Faculty of Science, Tanta University, Egypt | ||||
2Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Kafrelsheikh University, Egypt | ||||
Abstract | ||||
This study was conducted to isolate and characterize the major histocompatibility complex (MHC) class IIA gene from Nile tilapia and to compare between different genotypes of this gene following infection with Aeromonas hydrophila. To achieve our goals PCR was used to isolate MHC IIA gene following extraction of genomic DNA from fish blood and then single strand conformational polymorphism (SSCP) was used to genotype resistant and susceptible/diseased fish. The results showed 12 nucleotides deletion polymorphisms in MHC IIA with a product size of 267 bp in control and resistant fish and 255 bp in susceptible/diseased fish(Glover KA, et al.2007) .Additionally , sequences out of 50 from susceptible/diseased fish showed a nonsynonymous G23T SNP which interestingly changed the stop codon to glycin (G) amino acid with subsequent right shift to the open reading frame (ORF) . This SNP also resulted in three genotype (TT ,TG ,GG) with frequencies of 0.9,0.07 and 0.03 respectively . total protein , albumin and globulin as well as in the phagocytic. The G23T SNP was also associated with the expression level of MHC IIA in spleen with lowest expression in susceptible/diseased fish carrying GG genotype to resistant fish (Xu T et al .,2008). Thus , SNP-disease resistance association analysis revealed that genotypes (GG and TG) and alleles (G) of the G23T SNP were high risk for infection | ||||
Keywords | ||||
Major histocompatibility complex; Genotype; Nile tilapia; SSCPPCR | ||||
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