Study the Hemolytic and Cytotoxic Effects of Saponin Isolated from Soy Bean. | ||||
| Delta Journal of Science | ||||
| Article 3, Volume 38, Issue 2, December 2017, Page 150-160 PDF (1.52 MB) | ||||
| Document Type: Research and Reference | ||||
| DOI: 10.21608/djs.2017.139440 | ||||
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| Authors | ||||
| Mai M. El-Keiy; Aliaa M. Radwan* ; Tarek M. Mohamed | ||||
| Department of Chemistry, Fac. of Science, Tanta University, Tanta, Egypt | ||||
| Abstract | ||||
| Saponin is an important class of natural products that can be found primarily in many plants, as well as in some marine animals. It has a diverse range of properties, which include pharmacological and medicinal properties, strong hemolytic properties, as well as antimicrobial, insecticidal, and anticancer activities. Our study aimed to isolate the saponin from different plant sources (legumes) and to evaluate its hemolytic and cytotoxic activity in higher plant source. In this study the saponin was extracted from legumes using choloroform and ethanol. The concentration of saponin was determined by colorimetric method. Soybean saponin source was loaded on silica gel column chromatography and was detected by thin layer chromatography (TLC), infrared spectroscopy (IR) and confirmed by high performance liquid chromatography (HPLC). In addition the hemolytic activity of soybean saponin was evaluated on human blood erythrocyte and the cytotoxic effect on human colon cancer cell line (Coca) was determined by using MTT assay. Our result showed the presence of blue violet spot on thin layer chromatography in soya been saponin and confirmed by IR spectrum by presence of C-H bond of at 2927cm-1, CO stretching at 1059 cm-1,C=C bond at 1628cm-1 and OH bond at 3400cm-1. Hemolytic dose (HD50) that made hemolysis for 50% of erythrocyte was 0.412 mg /ml. MTT assay releaved that saponin extracted from soya bean had IC50 43.4μg/ml against colon cancer cell line in 48hr treatment. In conclusion, Soya saponin inhibits the proliferation of colon cancer and thus it may have therapeutic application in future. | ||||
| Keywords | ||||
| soybean; saponin; hemolysis; colon cancer | ||||
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