Detection of mutation in Exon 2-3 in Perforin gene and Exon4 in Fas gene in sample leukemia Iraqi patients
Ahmed, N., Hamza, S., Al-Faisal, A., Yousaf, W., Ghareeb, A. (2013). Detection of mutation in Exon 2-3 in Perforin gene and Exon4 in Fas gene in sample leukemia Iraqi patients. EKB Journal Management System, 36(2), 61-67. doi: 10.21608/djs.2013.139648
Najwa Shihab Ahmed; Subhi Jawad Hamza; Abdul Hussen Al-Faisal; Wan Mohtar Yousaf; Abdulameer M Ghareeb. "Detection of mutation in Exon 2-3 in Perforin gene and Exon4 in Fas gene in sample leukemia Iraqi patients". EKB Journal Management System, 36, 2, 2013, 61-67. doi: 10.21608/djs.2013.139648
Ahmed, N., Hamza, S., Al-Faisal, A., Yousaf, W., Ghareeb, A. (2013). 'Detection of mutation in Exon 2-3 in Perforin gene and Exon4 in Fas gene in sample leukemia Iraqi patients', EKB Journal Management System, 36(2), pp. 61-67. doi: 10.21608/djs.2013.139648
Ahmed, N., Hamza, S., Al-Faisal, A., Yousaf, W., Ghareeb, A. Detection of mutation in Exon 2-3 in Perforin gene and Exon4 in Fas gene in sample leukemia Iraqi patients. EKB Journal Management System, 2013; 36(2): 61-67. doi: 10.21608/djs.2013.139648

Detection of mutation in Exon 2-3 in Perforin gene and Exon4 in Fas gene in sample leukemia Iraqi patients

Delta Journal of Science
Article 10, Volume 36, Issue 2, June 2013, Page 61-67  XML PDF (1.55 MB)
Document Type: Research and Reference
DOI: 10.21608/djs.2013.139648
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Authors
Najwa Shihab Ahmed1; Subhi Jawad Hamza2; Abdul Hussen Al-Faisal3; Wan Mohtar Yousaf4; Abdulameer M Ghareeb3
1Biotechnology Research Center, Al-Nahrain University, Baghdad, Iraq.
2Baghdad University, Science college, Biotechnology
3engineering and biotechnology institute, Baghdad University
4University Kebangsaan Malaysia, Faculty of Science and Technology, School of Bioscience
Abstract
This work aimed to study the possible mutations in Perforin gene (PRF1) exon 2-3 and Fas
gene exon 4 in Acute Lymphocytic Leukemia (ALL) and Chronic Lymphocytic Leukemia
(CLL) patients from Iraq. In an attempt to detect any mutation within PRF1 gene and Fas genes,
a sequencing analysis for these genes were made. The results were alignment with sequences
present in the Gene Bank seeking for homology and differences. A DNA sequence for Homo
sapiens PRF1 gene was found compatible with genes of ALL, CLL patients and healthy
controls, 100% compatibility was found in the flank DNA sense and antisense sequences from
healthy. However, 99% compatibility was detected for the genes isolated from ALL patients
with an insertion of C697 and A698 G in the flank DNA sense strand and insertion of G697 and
T698C in flank DNA antisense strand of the gene. Morever, 99% compatibility was detected for
the genes isolated from CLL patients with two transition mutations in the flank DNA sense
strand of C957T and C1035T and one transition mutation in the flank DNA antisense strand of
G957A. However, no mutations were detected in Fas gene isolated from ALL, CLL, and healthy
controls.
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