Purification and kinetic studies of bovine kidney cyclooxygenases (COXs) | ||||
| Delta Journal of Science | ||||
| Article 7, Volume 37, Issue 1, June 2016, Page 65-62 PDF (1.33 MB) | ||||
| Document Type: Research and Reference | ||||
| DOI: 10.21608/djs.2016.139920 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Raef S. Shams1; Rasha Abu-Khudir2; Ehab M. M. Ali3 | ||||
| 11Biochemistry division, Chemistry department, Faculty of Science, Tanta University, Tanta, Egypt. | ||||
| 2Biochemistry division, Chemistry department, Faculty of Science, Tanta University, Tanta, Egypt. | ||||
| 3Biochemistry Division, Chemistry department, Faculty of Science, Tanta University, Tanta, Egypt. | ||||
| Abstract | ||||
| Cyclooxygenases (EC 1.14.99.1) is enzyme family that produces inflammatory prostaglandins, also known as prostaglandin H synthase (PGHS) or prostaglandin-endoperoxide synthase, is expressed in several tissues. COX activity has been detected in both brain and kidney of mice and bovine kidney (that showed the highest activity). Optimum biochemical properties of enzyme activity including incubation time, enzyme and substrate concentrations and pH were determined. Kinetic parameters of COX activity were indicated that the Vmax and Km values were of 66.66 μmole/min and 125.73 μM-1, respectively. COX has been purified from bovine kidney by detergent solubilization, (NH4)2SO4 precipitation, dialysis and ion exchange chromatography on DEAE-sepharose. Three isoforms were obtained (coxI, coxII and coxIII) that showed specific activities of 592.27, 1323.3 and 1825 U/mg protein and 0.72, 1.6 and 2.2 folds purification over the crude homogenate, respectively. The purified enzyme exhibited a single protein band on Coomassie Brilliant Blue stained SDS-PAGE gel corresponding a molecular weight 70 KDa. | ||||
| Keywords | ||||
| Prostaglandins; Cyclooxygenase; Arachidonic acid; Inflammation | ||||
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