Evaluation of the Role Of bla Genes in Beta Lactam and Methicillin Resistant Staphylococcus aureus | ||||
| Egyptian Journal of Botany | ||||
| Article 3, Volume 59, Issue 1, April 2019, Page 29-38 PDF (640.94 K) | ||||
| Document Type: Regular issue (Original Article) | ||||
| DOI: 10.21608/ejbo.2018.4221.1187 | ||||
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| Authors | ||||
Sarah H. Ahmed  1; Sahar T.M. Tolba1; Yehia A. Al Zawahry2
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| 1Department of Microbiology, Faculty of science -Ain-Shams University ,Cairo, Egypt | ||||
| 2Department of Botany, Zagazig University, Zagazig, Egypt | ||||
| Abstract | ||||
| One hundred and nineteen clinical samples were isolated from patients admitted in different hospitals in El-Sharkia Governorate. Sixty six isolates were confirmed to be S. aureus. Susceptibility to different antimicrobial agents and Minimum inhibitory concentration tests showed that all the isolates were resistant to 𝛽-lactam antibiotics, 77.2% (n= 51) isolates were methicillin resistant S. aureus MRSA, while almost all the isolates were sensitive to vancomycin and tigecycline. Polymerase chain reaction (PCR) of mecA, encoding methicillin resistance, and blaZ, β-lactamase biosynthetic gene, revealed the coexistence of both genes in 56.8% (n= 29/51) of the isolates. Meanwhile, 11.7% (n= 6/51) of MRSA isolates phenotypically resistant to oxacillin were found to be mecA-. This data support the fact that the expression of bla genes enhanced the phenotypic expression of oxacillin resistance as a result of β-lactamase hyperproduction. On the other hand, 33% of MRSA (n=17/51) were blaZ– suggesting a mutation event in blaZ or the existence of an alternative mechanisms for 𝛽-lactam resistance that may compete with mecA gene. | ||||
| Keywords | ||||
| Staphylococcus aureus; mec A; blaZ; MRSA; MIC- β-lactamase resistant MRSA | ||||
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