Histological and Immunohistochemical study on the protective effect of Nano-selenium against experimental toxicity induced by Aflatoxin B1 (AFB1) on the gingiva and periodontal ligament of albino rats
Hamed, G., Selim, M. (2021). Histological and Immunohistochemical study on the protective effect of Nano-selenium against experimental toxicity induced by Aflatoxin B1 (AFB1) on the gingiva and periodontal ligament of albino rats. EKB Journal Management System, 67(Issue 1 - January (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)), 357-366. doi: 10.21608/edj.2020.47103.1298
Ghada Mohammed said Hamed; Manar AbdelAziz Selim. "Histological and Immunohistochemical study on the protective effect of Nano-selenium against experimental toxicity induced by Aflatoxin B1 (AFB1) on the gingiva and periodontal ligament of albino rats". EKB Journal Management System, 67, Issue 1 - January (Oral Medicine, X-Ray, Oral Biology & Oral Pathology), 2021, 357-366. doi: 10.21608/edj.2020.47103.1298
Hamed, G., Selim, M. (2021). 'Histological and Immunohistochemical study on the protective effect of Nano-selenium against experimental toxicity induced by Aflatoxin B1 (AFB1) on the gingiva and periodontal ligament of albino rats', EKB Journal Management System, 67(Issue 1 - January (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)), pp. 357-366. doi: 10.21608/edj.2020.47103.1298
Hamed, G., Selim, M. Histological and Immunohistochemical study on the protective effect of Nano-selenium against experimental toxicity induced by Aflatoxin B1 (AFB1) on the gingiva and periodontal ligament of albino rats. EKB Journal Management System, 2021; 67(Issue 1 - January (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)): 357-366. doi: 10.21608/edj.2020.47103.1298

Histological and Immunohistochemical study on the protective effect of Nano-selenium against experimental toxicity induced by Aflatoxin B1 (AFB1) on the gingiva and periodontal ligament of albino rats

Egyptian Dental Journal
Article 6, Volume 67, Issue 1 - January (Oral Medicine, X-Ray, Oral Biology & Oral Pathology) - Serial Number 3, January 2021, Page 357-366  XML PDF (2.57 MB)
Document Type: Original Article
DOI: 10.21608/edj.2020.47103.1298
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Authors
Ghada Mohammed said Hamed email orcid 1; Manar AbdelAziz Selimorcid 2
1ORAL BIOLOGY, SUZE CANAL UNVERSITY
2Oral Biology,Faculty of Dentistry,Suez Canal University, Ismailia,Egypt
Abstract
This current study was conducted to evaluate the ability of Nano-selenium (Nano Se) in inhibition of aflatoxin B1 toxicity on gingiva and periodontal ligament of rats through histological investigation and immunohistochemical detection of TNF-α & caspase-3 expression in gingiva and desmin expression in the periodontal ligament. Material and Methods: Forty adult male albino rats were divided into 4 equal groups: Group I served as control rats (normal), Group II (aflatoxin B1 group) received aflatoxin B1 (250 μg/kg b.w/day) orally by gastric tube 5 days/week for 4 weeks, Group III (AFB1and Nano-Se group) received aflatoxin B1 (250 μg/kg b.w./day) and 100 mg /animal of Nano-Se orally for 5 days/week 4 weeks. Group IV (Nano-Se group) received 100 mg /animal of Nano-Se orally for 5 days/week through gastric tube. At the end of the experiment, the upper and lower jaws were dissected out after scarification under general anesthesia. Gingival and jaw samples were collected and processed for histological techniques. Immunohistochemical estimation of TNF-α as pro-inflammatory mediator, caspase-3 to determine any apoptotic cells and desmin for detection of collagen fibers was performed
Keywords
aflatoxin B1; nano selenium; TNF-α; caspase-3; desmin
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