Effect of Dietary Inclusion of Black Cumin (Nigella sativa) Seeds on Histological Parameters of Caecal Tonsils and Bursa of Fabricius in Broiler Chickens
Hamedi, S., Shomali, T., Molayemvand, P. (2015). Effect of Dietary Inclusion of Black Cumin (Nigella sativa) Seeds on Histological Parameters of Caecal Tonsils and Bursa of Fabricius in Broiler Chickens. EKB Journal Management System, 6(2), 33-39. doi: 10.21608/eajbsd.2015.14110
Somayeh Hamedi; Tahoora Shomali; Pooya Molayemvand. "Effect of Dietary Inclusion of Black Cumin (Nigella sativa) Seeds on Histological Parameters of Caecal Tonsils and Bursa of Fabricius in Broiler Chickens". EKB Journal Management System, 6, 2, 2015, 33-39. doi: 10.21608/eajbsd.2015.14110
Hamedi, S., Shomali, T., Molayemvand, P. (2015). 'Effect of Dietary Inclusion of Black Cumin (Nigella sativa) Seeds on Histological Parameters of Caecal Tonsils and Bursa of Fabricius in Broiler Chickens', EKB Journal Management System, 6(2), pp. 33-39. doi: 10.21608/eajbsd.2015.14110
Hamedi, S., Shomali, T., Molayemvand, P. Effect of Dietary Inclusion of Black Cumin (Nigella sativa) Seeds on Histological Parameters of Caecal Tonsils and Bursa of Fabricius in Broiler Chickens. EKB Journal Management System, 2015; 6(2): 33-39. doi: 10.21608/eajbsd.2015.14110

Effect of Dietary Inclusion of Black Cumin (Nigella sativa) Seeds on Histological Parameters of Caecal Tonsils and Bursa of Fabricius in Broiler Chickens

Egyptian Academic Journal of Biological Sciences, D. Histology & Histochemistry
Article 3, Volume 6, Issue 2, December 2015, Page 33-39  XML PDF (405.3 K)
Document Type: Original Article
DOI: 10.21608/eajbsd.2015.14110
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Authors
Somayeh Hamedi1; Tahoora Shomali2; Pooya Molayemvand3
1Department of Basic Sciences, Faculty of Veterinary Medicine, Islamic Azad University, Karaj Branch, Alborz, Iran.
2Division of Pharmacology and Toxicology, Department of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
3Department of Basic Sciences, Faculty of Veterinary Medicine, Islamic Azad University, Karaj Branch, Alborz, Iran
Abstract
This study aimed to evaluate the effect of long term administration of Nigella sativa seed as an herbal immunomodulatory agent on histological features of caecal tonsils and bursa of Fabricius in broiler chickens. Fifty, one-day old chick were randomly divided into five equal groups and fed with diets containing  0, 0.5, 1, 1.5, and 2% of N. sativa seedsfor 45 days. Then birds were slaughtered and 6μm-thick cross sections were made from caecal tonsils and bursa of Fabricius and stained with hematoxylin and eosin. Villus height and villus basal width, nodular unit width and height, follicular width and muscular layer width in caecal tonsils as well as height of plicae, follicular width, thickness of follicular cortex and medulla in bursa were determined. In addition, number of follicles in each plica in bursa of Fabricius and number of follicles per nodular unit in caecal tonsils were determined under light microscope. The results showed a change in all histomorphometric parameters of caecal tonsils due to N. sativa administration which followed a dose-dependent pattern in most cases. Villus height, nodular unit width and muscular layer width were decreased while other parameters increased as compared to control. Although all parameters in bursa increased due to N. sativa seed administration, plica height was the only parameter which showed a dose-dependent change. In conclusion, dietary inclusion of N .sativa especially at 2% dose, can improve immune structures of both bursa of Fabricius and caecal tonsils of broilers.
Keywords
Broilers; Bursa of Fabricius; Caecal tonsil; histology; Nigella Sativa
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