Flaxseed Oil Supplementation Afford Comparable Therapeutic effect to Metformin in Bleomycin Induced Pulmonary Fibrosis Rat model | ||||
| Bulletin of Egyptian Society for Physiological Sciences | ||||
| Article 2, Volume 41, Issue 3, July 2021, Page 296-315 PDF (958.49 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/besps.2020.38360.1071 | ||||
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| Authors | ||||
Eman Kolieb1; Shymaa Maher2; Karima El-Sayed   3
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| 1Physiology Department, Suez Canal University, Ismailia, 41522, Egypt. | ||||
| 2Biochemistry Department, Suez Canal University, Ismailia, 41522, Egypt. | ||||
| 3Physiology department, faculty of medicine, Suez canal university | ||||
| Abstract | ||||
| Background: Pulmonary Fibrosis is a chronic lung disease characterized by scar formation and respiratory insufficiency. Metformin is an antidiabetic drug that has recently been shown to prevent lung fibrosis via NADPH oxidase-4 suppression. Flaxseed oil has been shown to be a potent antioxidant that attenuates oxidative damage by inhibition of lipid peroxidation and the proinflammatory cytokine production of IL-6 and TNF-alpha. Aim: To investigate whether metformin and/or flaxseed oil could protect against bleomycin-induced pulmonary fibrosis and if this protection is mediated by inhibiting NOX-4 signaling pathway and the synthesis and release of inflammatory and oxidative stress markers. Material and Methods: Fifty adult male rats were randomized into 5 groups/10 rats each.Pulmonary fibrosis was induced by Bleomycin, delivered intratracheally at a concentration of 1.5mg/kg body weight once in group PF, MET, Flax and combined MET& Flax. Both metformin and Flaxseed oil were administered orally for 21 day after induction of PF. Withdrawal of blood samples for chemical and spectral assay of antioxidant markers was done. Histopathology and assessment of inflammatory and fibrotic markers and PCR expression of NOX4 gene were done. Results: There was a significant reduction in inflammatory cell count, histopathological score of inflammation and fibrosis in the three treatment groups. Also there was marked improvement in inflammatory (IL-6, TNF-a) and fibrotic markers (TGF-B and Col-1) in metformin group rather than flaxseed and combined group.Nox4 gene expression was significantly reduced in metformin treated group rather than other 2 groups | ||||
| Keywords | ||||
| Pulmonary fibrosis; metformin; flaxseed oil; bleomycin; NOX4 | ||||
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