Antibacterial and antifungal activity of chitosan against Bacillus cereus and Aspergillus niger isolated from some Egyptian canned and fast food | ||||
Delta Journal of Science | ||||
Article 9, Volume 42, Issue 1, December 2020, Page 90-99 PDF (1.84 MB) | ||||
Document Type: Research and Reference | ||||
DOI: 10.21608/djs.2020.142854 | ||||
View on SCiNiTO | ||||
Authors | ||||
Saida Mohamed Amer1; Azza Abd El-Rahman Mostafa2; Maha Mahmoud Azab1; Mostafa Fathi Shaaban1 | ||||
1Botany and Microbiology Department, Faculty of Science, Tanta University, Tanta, Egypt. | ||||
2Biological and Environmental Departmental Science, Faculty of Home Economics, Al-Azhar University, Tanta, Egypt. | ||||
Abstract | ||||
A total of (213) canned food samples comprising of Tuna &Sardines, Juices, Tomatoes pasts, Jam and Beef were randomly collected from super stores and local markets in Tanta city from Awlad Ragab, Fatthallah, Munshwi, Casion. Also 24 fast food samples collected from local cafeterias and restaurants in Tanta city from Al Gaan, Abu Deshish, Al Baraka, Abu Owaf. All canned food samples were within expiry date, none of which is bloated, leaking and/or physically damaged. Samples were investigated for some bacteria and fungi using specific media and incubated for suitable incubation period. The results revealed that species of microbes isolated in this study namely Bacillus subtilis, Bacillus cereus, Bacillus atrophaeus., Staphylococcus saprophyticus, Enterococcus faecalis, Staphylococcus epidermides, E. coli, Klebsiella, Salmonella , Aspergillus niger, Penicillium notatum, Candida tropicalis and Saccharomyces cerevisiae. Biochemical tests were performed for all isolates to know the most common isolates (Bacillus cereus and Aspergillus niger). Antimicrobial activities of chitosan were investigated against the most common isolates B. cereus and A. niger. Results revealed that chitosan has antibacterial activity towards Bacillus cereus. The minimum inhibition concentration (MIC) for chitosan was 6.25 μg/ml with mean diameter of inhibition zone 8 mm. Also chitosan has antifungal activity toward Aspergillus.niger with minimum inhibition concentration (MIC) 30mg/ml and percent of fungal growth inhibition 16.7%. | ||||
Keywords | ||||
Canned food; Fast food; Aspergillus niger; Bacillus cereus; Biochemical tests; Minimum inhibition concentration | ||||
Statistics Article View: 138 PDF Download: 351 |
||||