Effect of L-Ascorbic Acid and Alpha-Tocopherol on The Monosodium Glutamate-induced Neurobehavioral Changes in Rats | ||||
| Bulletin of Egyptian Society for Physiological Sciences | ||||
| Article 10, Volume 41, Issue 3, July 2021, Page 410-427 PDF (1.01 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/besps.2021.47301.1082 | ||||
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| Authors | ||||
Safaa Mohamed El-kotb Saleh1; Omnia Ameen 2; Marwa Salah Gadallah3; Aya Saleh Abd Elaziz Saleh  2; Hesham Ahmed Diaa Abdel-Razek 4
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| 1Medical Physiology Department, Faculty of Medicine, Menoufia University, Shebein El-Koum, Egypt. | ||||
| 2Medical Physiology Department, Faculty of Medicine, Menoufia University, Shebein El-Koum, Menoufia Governorate, Egypt. | ||||
| 3Pathology Department, Faculty of Medicine, Menoufia University, Shebein El-Koum, Menoufia Governorate, Egypt. | ||||
| 4Medical Physiology Department, Faculty of Medicine, Menoufia University, Shebin El-Koum, Menoufia Governorate, Egypt | ||||
| Abstract | ||||
| Background: Monosodium Glutamate (MSG) is one of the most commonly used flavor-enhancing substances that may lead to neurological disorders. Objectives: The present work aimed to evaluate the role of L-ascorbic acid (AA) and -tocopherol (T) on the MSG-induced memory and neurobehavioral changes in rats. Methods: Thirty male Wistar albino rats were randomized into five equal groups: (1) control group, (2) MSG group received MSG (2mg/g BW) daily, (3) MSG+A group received MSG as in MSG group, and AA (100 mg/kg BW) daily, (4) MSG+T group received MSG as in MSG group, and T (600 mg/kg BW) twice weekly, and (5) MSG+AT group received MSG as in MSG group, AA as in MSG+A group, and T as in MSG+T group. After 3 weeks, neurobehavioral changes were assessed by open field test and Y maze. Oxidative stress markers were estimated, and immunohistochemistry was studied in hippocampal region. Results: MSG resulted in impairment of memory and induction of anxiety, with increased hippocampal malondialdehyde and decreased superoxide dismutase and glutathione peroxidase. Treatment with AA or T improved all the measured biochemical parameters, and the MSG-induced hippocampal degenerative changes, with decreased glial fibrillary acidic protein (GFAP) and synaptophysin expression. Combined administration of both vitamins was more effective in amelioration of MSG-induced impairments rather than taking AA or T alone. Conclusion: Both AA and T exhibit protective effects against neurobehavioral changes, oxidative stress and hippocampal degenerative changes induced by MSG toxicity, with more potent efficacy of their combination. | ||||
| Keywords | ||||
| monosodium glutamate; L-ascorbic acid; alpha-tocopherol; GFAP; synaptophysin | ||||
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