The Efficiency of Kisspeptin and GnRH as Stimulators of Gonadotrophins and Testosterone in Prepubertal Male Cattle
Ahmed, A., Haridy, M., Kassab, A., Ahmed, H., Senosy, W., Toh-Ichi, H., Tsutomu, H. (2018). The Efficiency of Kisspeptin and GnRH as Stimulators of Gonadotrophins and Testosterone in Prepubertal Male Cattle. EKB Journal Management System, 46(2), 136-145. doi: 10.21608/zvjz.2018.14386
Ahmed E. Ahmed; Mohie Haridy; Ayman Y. Kassab; Hassan Ahmed; Waleed Senosy; Hirata Toh-Ichi; Hashizume Tsutomu. "The Efficiency of Kisspeptin and GnRH as Stimulators of Gonadotrophins and Testosterone in Prepubertal Male Cattle". EKB Journal Management System, 46, 2, 2018, 136-145. doi: 10.21608/zvjz.2018.14386
Ahmed, A., Haridy, M., Kassab, A., Ahmed, H., Senosy, W., Toh-Ichi, H., Tsutomu, H. (2018). 'The Efficiency of Kisspeptin and GnRH as Stimulators of Gonadotrophins and Testosterone in Prepubertal Male Cattle', EKB Journal Management System, 46(2), pp. 136-145. doi: 10.21608/zvjz.2018.14386
Ahmed, A., Haridy, M., Kassab, A., Ahmed, H., Senosy, W., Toh-Ichi, H., Tsutomu, H. The Efficiency of Kisspeptin and GnRH as Stimulators of Gonadotrophins and Testosterone in Prepubertal Male Cattle. EKB Journal Management System, 2018; 46(2): 136-145. doi: 10.21608/zvjz.2018.14386

The Efficiency of Kisspeptin and GnRH as Stimulators of Gonadotrophins and Testosterone in Prepubertal Male Cattle

Zagazig Veterinary Journal
Article 6, Volume 46, Issue 2, June 2018, Page 136-145  XML PDF (789.34 K)
Document Type: Original Article
DOI: 10.21608/zvjz.2018.14386
View on SCiNiTO View on SCiNiTO
Authors
Ahmed E. Ahmed email 1; Mohie Haridy2; Ayman Y. Kassab3; Hassan Ahmed4; Waleed Senosy5; Hirata Toh-Ichi6; Hashizume Tsutomu6
1Theriogenology Department, Faculty of Veterinary Medicine, South Valley University, Qena 83523, Egypt
2Pathology and Clinical Pathology Department, Faculty of Veterinary Medicine, South Valley University, Qena 83523, Egypt
3Animal Production Department, Faculty of Agriculture, Assiut University, New valley 51714, Egypt
4Physiology Department, Faculty of Veterinary Medicine, South Valley University, Qena 83523, Egypt
5Theriogenology Department, Faculty of Veterinary Medicine, Assiut University, New valley 51714, Egypt
6Animal Science Department, Faculty of Agriculture, Iwate University, Morioka 020-8550, Japan
Abstract
   Our study aimed to compare between the efficiency of kisspeptin-10 (Kp10) and gonadotropin-releasing hormone (GnRH) as a trigger stimulator for gonadotropins; luteinizing hormone (LH), follicle-stimulating hormone (FSH) and testosterone (T) in male calves. Four prepubertal male Japanese Black calves were used. The animals were given GnRH or Kp10 separately. Plasma LH and FSH concentrations were measured by radioimmunoassay (RIA) and T concentrations were assayed by enzyme-linked immunosorbent assay (ELISA). Gonadotropins were significantly increased in response to injections of GnRH and Kp10. However, the response of gonadotropins-release was significantly greater to GnRH than to Kp10 (P<0.05) throughout the 180-min period. Plasma T concentrations increased significantly following injection of Kp10 or GnRH (P<0.05) in comparison with the pre-injection levels (0.75 - 0.47 ng/ml) but no significant difference was observed in T-releasing in response to GnRH and Kp10 throughout the 180-min period. Histopathologically, the testicular tissue had seminiferous cords, essentially without lumina, rather than seminiferous tubules. The basal lamina was very thick and surrounded the immature ill-developed Sertoli as well as spermatogonia cells. Neither spermatid cells nor sperms were seen (no spermatogenesis) with abundant interstitial tissue. No differences in structure of testes in Kp10 or GnRH-injected claves and those of control. Conclusively, GnRH and kisspeptin stimulated equally the secretion of T in prepubertal bulls but did not affect process of spermatogenesis.  
Keywords
Kisspeptin; GnRH; LH; FSH; testosterone
Statistics
Article View: 279
PDF Download: 323