Morphological, Ultrastructural, and Molecular Characterization of Sarcocystis tenella from Sheep in Qena Governorate, Upper Egypt | ||||
| Egyptian Academic Journal of Biological Sciences, E. Medical Entomology & Parasitology | ||||
| Article 2, Volume 10, Issue 1, June 2018, Page 11-19 PDF (957.9 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/eajbse.2018.14456 | ||||
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| Authors | ||||
| Nermean M. Hussein1; Amal A. Hassan2; Osama H. Abd Ella3 | ||||
| 1Zoology Department, Faculty of Science, South Valley University, Qena, Egypt | ||||
| 2Zoology Department, Faculty of Science, Damanhour University, Egypt | ||||
| 3Medical Parasitology Department, Faculty of Medicine, South Valley University | ||||
| Abstract | ||||
| Small parts of esophagus and cardiac muscles of abattoir sheep from Qena, Upper Egypt between June 2016 to May 2017 were examined by both light microscope (LM), transmission electron microscope (TEM), and DNA sequence analysis of 18 S rRNA gene to determine the rate of infection with Sarcocystis spp. and identify the parasite according to morphological, ultrastructure characteristics, and DNA sequence analysis. LM and TEM studies revealed that microscopic, thin walled sarcocysts were detected in 47 of 63 male (74.6 %) and in 32 of 41 female (65.07 %) as a spindle-shaped with size (225 – 431.3 μm x 34.7- 82.4 μm) in esophagus muscles, and ovoid with size (141.6- 263 μm x 51.4- 82.3 μm) in cardiac muscles. The result sequences were compared with other previously sequenced Sarcocystis species retrieved from Gene Bank it was most closely refered to Sarcocystis tenella (identity 99- 100%). | ||||
| Keywords | ||||
| sheep; Sarcocystis tenella; TEM; 18 S rRNA gene; DNA sequence analysis; Egypt | ||||
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