Characterization of Cockroach Allergens as A factor of Dermatitis in Egypt | ||||
| Egyptian Academic Journal of Biological Sciences, E. Medical Entomology & Parasitology | ||||
| Article 1, Volume 8, Issue 1, December 2016, Page 1-11 PDF (709.39 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/eajbse.2016.14483 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Bouthaina A. M1; Magda H. Radi1; Weam M. Kamal2 | ||||
| 11-Entomology Department, Faculty of Science, Ain Shams University, Egypt | ||||
| 2Faculty of Medicine, Al Azhar University, Egypt | ||||
| Abstract | ||||
| Cockroach have been recognized as a major cause of asthma in urban environment as the Egyptian randomized areas. Extraction, characterization, and detecting immune response to such allergens were determined. Cockroach allergens proteins were characterized using SDS-PAGE analysis which yield protein fractions of molecular weights ranging from 190-35 KD, while amino acid analysis proved the presence of glycine, cystine, glutamic acid and aspartic acid which sharing with the commercial cockroach extract. HPLC analysis also revealed that there were many peaks closely the same in retention time between commercial and prepared cockroach allergens. Antigen antibody reaction revealed positive reaction to both commercial and local prepared cockroach allergens with human serum samples from persons had highly detectable total IGE-Ab. | ||||
| Keywords | ||||
| Cockroach allergens; dermatitis; Egypt | ||||
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