OPTIMIZATION OF A CULTURE MEDIUM FOR BIOMASS AND δ-ENDOTOXIN PRODUCTION BY A RECOMBINANT ESCHERICHIA COLI STRAIN
Gamal, R., R., A., Mohammed, A., El-Sawy, M. (2008). OPTIMIZATION OF A CULTURE MEDIUM FOR BIOMASS AND δ-ENDOTOXIN PRODUCTION BY A RECOMBINANT ESCHERICHIA COLI STRAIN. EKB Journal Management System, 16(1), 41-55. doi: 10.21608/ajs.2008.14535
Rawia F. Gamal; Al-Fateh S R.; Abeer M. Mohammed; M. El-Sawy. "OPTIMIZATION OF A CULTURE MEDIUM FOR BIOMASS AND δ-ENDOTOXIN PRODUCTION BY A RECOMBINANT ESCHERICHIA COLI STRAIN". EKB Journal Management System, 16, 1, 2008, 41-55. doi: 10.21608/ajs.2008.14535
Gamal, R., R., A., Mohammed, A., El-Sawy, M. (2008). 'OPTIMIZATION OF A CULTURE MEDIUM FOR BIOMASS AND δ-ENDOTOXIN PRODUCTION BY A RECOMBINANT ESCHERICHIA COLI STRAIN', EKB Journal Management System, 16(1), pp. 41-55. doi: 10.21608/ajs.2008.14535
Gamal, R., R., A., Mohammed, A., El-Sawy, M. OPTIMIZATION OF A CULTURE MEDIUM FOR BIOMASS AND δ-ENDOTOXIN PRODUCTION BY A RECOMBINANT ESCHERICHIA COLI STRAIN. EKB Journal Management System, 2008; 16(1): 41-55. doi: 10.21608/ajs.2008.14535

OPTIMIZATION OF A CULTURE MEDIUM FOR BIOMASS AND δ-ENDOTOXIN PRODUCTION BY A RECOMBINANT ESCHERICHIA COLI STRAIN

Arab Universities Journal of Agricultural Sciences
Article 2, Volume 16, Issue 1, March 2008, Page 41-55  XML PDF (724.82 K)
Document Type: Original Article
DOI: 10.21608/ajs.2008.14535
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Authors
Rawia F. Gamal* 1; Al-Fateh S R.2; Abeer M. Mohammed2; M. El-Sawy1
1Dept. Microbiol., Fac. Agric., Ain Shams University, Shobra El-Khema, Cairo, Egypt
2Plant Protection Research Institute, Agric. Research Center, Dokki, Giza, Egypt
Abstract
A recombinant strain of Escherichia coli harboring a plasmid containing the Bacillus thuringiensis δ-endotoxin synthesis gene, was tested for its efficacy to synthesize δ-endotoxin, in a complex medium containing sucrose and yeast extract. Also, the recombinant E. coli strain was tested for its efficacy against the 2nd instars of Spodoptera littoralis. The recombinant strain of E. coli showed a good activity against the 2nd instars of S. littoralis, the mortality was 70 % after 7 days at room temperature. A high cell biomass (8.8gL-1) and δ-endotoxin concentration (6.8 mg L-1), were obtained by the shake flask culture (100 ml medium/250 ml flask, at 200 rpm), of the recombinant E. coli in modified MR medium containing sucrose (20g/L), as carbon source and yeast extract as nitrogen source, in the presence of CaCO3, K2HPO4, MgSO4, FeSO4 and ZnSO4 as mineral salts. The best pH values for cell biomass production and endotoxin production were 7.0 and 7.5, respectively. The corresponding figures for the best temperature were 37oC and 30oC, respectively. The use of some byproducts such as black-strap molasses, corn-steep liquor and cheese whey, as an alternative for carbon and nitrogen sources of medium, were found to enhance the cell growth but showed no effect on endotoxin production.
Keywords
Recombinant Escherichia coli; Biomass; δ-endotoxin production; Medium optimization
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