FACTORS AFFECTING THE (PRODUCTION OF EXTRACELLULAR NEUTRAL PROTEASE) BY STREPTOMYCES MICROFLAVUS
M. Hassanein, S., M. Rifaat, H., Hamed El-Said, O., A. Saleh, S., S. M. Selim, M. (2009). FACTORS AFFECTING THE (PRODUCTION OF EXTRACELLULAR NEUTRAL PROTEASE) BY STREPTOMYCES MICROFLAVUS. EKB Journal Management System, 17(1), 33-41. doi: 10.21608/ajs.2009.14547
Saadia, M. Hassanein; Hala M. Rifaat; Osama Hamed El-Said; Souad A. Saleh; Manal S. M. Selim. "FACTORS AFFECTING THE (PRODUCTION OF EXTRACELLULAR NEUTRAL PROTEASE) BY STREPTOMYCES MICROFLAVUS". EKB Journal Management System, 17, 1, 2009, 33-41. doi: 10.21608/ajs.2009.14547
M. Hassanein, S., M. Rifaat, H., Hamed El-Said, O., A. Saleh, S., S. M. Selim, M. (2009). 'FACTORS AFFECTING THE (PRODUCTION OF EXTRACELLULAR NEUTRAL PROTEASE) BY STREPTOMYCES MICROFLAVUS', EKB Journal Management System, 17(1), pp. 33-41. doi: 10.21608/ajs.2009.14547
M. Hassanein, S., M. Rifaat, H., Hamed El-Said, O., A. Saleh, S., S. M. Selim, M. FACTORS AFFECTING THE (PRODUCTION OF EXTRACELLULAR NEUTRAL PROTEASE) BY STREPTOMYCES MICROFLAVUS. EKB Journal Management System, 2009; 17(1): 33-41. doi: 10.21608/ajs.2009.14547

FACTORS AFFECTING THE (PRODUCTION OF EXTRACELLULAR NEUTRAL PROTEASE) BY STREPTOMYCES MICROFLAVUS

Arab Universities Journal of Agricultural Sciences
Article 3, Volume 17, Issue 1, March 2009, Page 33-41  XML PDF (503 K)
Document Type: Original Article
DOI: 10.21608/ajs.2009.14547
View on SCiNiTO View on SCiNiTO
Authors
Saadia, M. Hassanein* 1; Hala M. Rifaat2; Osama Hamed El-Said3; Souad A. Saleh3; Manal S. M. Selim3
1- Microbiology Department, Ain Shams University, Abbasiya,Cairo, Egypt
2- Microbial Chemistry Department, National Research Centre, Dokki, Cairo, Egypt
3Microbial Biotechnology Department, National Research Centre, Dokki, Cairo, Egypt
Abstract
 
In order to produce neutral protease from Streptomyces microflavus, it was cultivated in basal medium containing soluble starch, potassium nitrate, dipotassium hydrogen phosphate, magnesium sulphate, sodium chloride, calcium carbonate and ferrous sulphate. Protease production increased with decreasing the ratio of culture broth to vessel volume 1:5 and shaking at 150 rpm. Protease production was low when pH was < 5 or > 9. The productivity of protease decreased sharply when the incubation temperature increased from 30 to 450C. The maximum yield of protease was obtained at the third day of incubation. Soluble starch and yeast extract were the best carbon and nitrogen sources, respectively. Supplementation with calcium carbonate enhanced protease production. In addition, the dipotassium hydrogen phosphate was the best phosphorous source. Para chloromercuribenzoic acid and phenyl methylsulfonylfloride had significant inhibitory effect on protease production.
Keywords
Streptomycetes microflavus; Cultivation; production; Protease enzyme
Statistics
Article View: 142
PDF Download: 227