Optimization of Aeration and Agitation for α-amylase production by Aspergillus flavus from water hyacinth | ||||
| International Journal of Industry and Sustainable Development | ||||
| Article 3, Volume 2, Issue 1, 2021, Page 25-35 PDF (536.26 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ijisd.2021.145562 | ||||
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| Authors | ||||
Enas Mostafa1; Mostafa Abo Elsoud   2; Marwa S. Salem3; Eman El-Tabakh4; Nagwa M. Sidkey4
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| 1chemical Engineering and Pilot Plant Department, National Research Centre, Egypt | ||||
| 2National Research Centre | ||||
| 3department of Botany and Microbiology, Faculty of Science, Al Azhar University (Girls Branch), Cairo, Egypt | ||||
| 4Department of Botany and Microbiology, Faculty of Science, Al Azhar University (Girls Branch), Cairo, Egypt | ||||
| Abstract | ||||
| Amylases are a group of commercially important enzymes with various clinical, medical and biotechnological applications. The current study aims to optimize the agitation and aeration (oxygen transfer) conditions for α-amylase production by Aspergillus flavus using water hyacinth extract; as an available waste and very cheap nutritional substance, in a lab scale stirred tank bioreactor (submerged fermentation). Data showed that the maximal cell biomass and enzyme specific activity were reached at low aeration rate (0.5 v/v/m) and moderate agitation speed (200 rpm). The maximum specific amylase production rate has been reached after 24 hrs. In addition, it has been confirmed that scaling-up Aspergillus flavus does not, inversely, affect α-amylase productivity compared with flask level production. Aspergillus flavus has been approved as promising fungal isolate for production of a-amylase using water hyacinth for application in many fields. | ||||
| Keywords | ||||
| α-amylase; Fermentation; Bioreactor; Aeration; Agitation; Water hyacinth | ||||
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