Bioguided Isolation and in-Silico Analysis of Hep-G2 Cytotoxic Constituents From Laurus nobilis Linn. Cultivated In Egypt
Nagah, N., Mostafa, I., Osman, A., Dora, G., El-Sayed, Z., Ateya, A. (2021). Bioguided Isolation and in-Silico Analysis of Hep-G2 Cytotoxic Constituents From Laurus nobilis Linn. Cultivated In Egypt. EKB Journal Management System, 64(5), 2731-2745. doi: 10.21608/ejchem.2021.55937.3197
Nesma Nagah; Islam Mostafa; Ali Osman; Gamal Dora; Zeinab El-Sayed; Abdel-Monem Ateya. "Bioguided Isolation and in-Silico Analysis of Hep-G2 Cytotoxic Constituents From Laurus nobilis Linn. Cultivated In Egypt". EKB Journal Management System, 64, 5, 2021, 2731-2745. doi: 10.21608/ejchem.2021.55937.3197
Nagah, N., Mostafa, I., Osman, A., Dora, G., El-Sayed, Z., Ateya, A. (2021). 'Bioguided Isolation and in-Silico Analysis of Hep-G2 Cytotoxic Constituents From Laurus nobilis Linn. Cultivated In Egypt', EKB Journal Management System, 64(5), pp. 2731-2745. doi: 10.21608/ejchem.2021.55937.3197
Nagah, N., Mostafa, I., Osman, A., Dora, G., El-Sayed, Z., Ateya, A. Bioguided Isolation and in-Silico Analysis of Hep-G2 Cytotoxic Constituents From Laurus nobilis Linn. Cultivated In Egypt. EKB Journal Management System, 2021; 64(5): 2731-2745. doi: 10.21608/ejchem.2021.55937.3197

Bioguided Isolation and in-Silico Analysis of Hep-G2 Cytotoxic Constituents From Laurus nobilis Linn. Cultivated In Egypt

Egyptian Journal of Chemistry
Article 49, Volume 64, Issue 5, May 2021, Page 2731-2745  XML PDF (994.58 K)
Document Type: Original Article
DOI: 10.21608/ejchem.2021.55937.3197
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Authors
Nesma Nagah1; Islam Mostafa email orcid 1; Ali Osmanorcid 2; Gamal Dora1; Zeinab El-Sayed1; Abdel-Monem Ateya1
1Department of Pharmacognosy, Faculty of Pharmacy, Zagazig University, Zagazig, Egypt.
2Department of Biochemistry, Faculty of Agriculture, Zagazig University, Zagazig, Egypt
Abstract
The air dried leaves of Laurus nobilis cultivated in Egypt were extracted by petroleum ether followed by methylene chloride then ethyl acetate and finally methanol. The preliminary cytotoxic screening of these extracts against Hep-G2 using vinblastine sulfate (IC50= 2.93 μg/ml) as standard showed that petroleum ether, methylene chloride and ethyl acetate extracts exhibited strong cytotoxic activity (IC50= 10.6, 5.96 and 3.80 μg/ml, respectively) while the methanolic extract showed moderate activity (IC50= 23.2 μg/ml). Bio-guided chromatographic isolation of L. nobilis extracts resulted in isolation of seven compounds identified as 1-tricosanol (1), reynosin (2), protocatechuic acid (3), vincetoxicoside B (4) and vitexin (5). chemical structures have been established on the basis of physical, chemical and spectroscopic methods (UV, IR, MS, 1H-NMR, 13C-NMR and HSQC) in addition to comparison with literature data and /or authentic samples. Reynosin (2) showed the most potent cytotoxic activity against Hep-G2 (IC50= 4.98 μg/ml) among other isolated compounds, while vitexin (5) showed the lowest activity (IC50= 219 μg/ml). Other compounds showed moderate to weak activity. The isolated compounds were docked to caspase3 to reveal their possible cytotoxic mechanism of action on the enzymatic level. Protein fraction obtained after 0.5 hr hydrolysis with papain showed moderate cytotoxic activity (IC50= 200 μg/ml) and that obtained after 3.5 hr hydrolysis exhibited strong antioxidant action (45 % inhibition at a concentration of 200 μg/ml using DPPH assay).
Keywords
Laurus nobilis, Cytotoxicity, Hep-G2; Reynosin, Molecular modeling, Protein hydrolysis, Antioxidant
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