TAXONOMIC EVALUATION USING POLLEN GRAIN SCULPTURE AND SEED COAT CHARACTERS OF 11 TAXA OF GENUS HIBISCUS (MALVACEAE) IN EGYPT | ||||
Arab Universities Journal of Agricultural Sciences | ||||
Article 9, Volume 19, Issue 1, March 2011, Page 143-152 PDF (914.11 K) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/ajs.2011.14637 | ||||
View on SCiNiTO | ||||
Authors | ||||
M.A El-Kholy* ; Kasem W.T.; Mabrouk A.S. | ||||
Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Nasr City, Egypt | ||||
Abstract | ||||
Pollen grain morphology and seed coat characters of 11 cultivars belonging to two species of genus Hibiscus (Family Malvaceae) namely H. esculentus (H. Abelmoschus) and H. sabdariffa were investigated. This study was carried out using light microscope (LM) and Scanning Electron Microscopy (SEM). Pollen morphology of this genus is fairly uniform. Generally radially symmetrical apolar, mostly spheroidal, pantoporate. Seed exomorphic characters revealed four types of ornamentations; reticulate, ocealate,foveolate andruminate. Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE) was employed to characterize those taxa. Thirty one bands of seed protein profiles have been constructed from the gel. The produced dendrograms were analyzed by STATISCA program using UPGMA clustering method showed a close affinity among the seven H. esculentus cultivars and the four H. sabdariffa cultivars. | ||||
Keywords | ||||
Pollen grains morphology; Hibiscus; seed coat; SDS-PAGE | ||||
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