Citicoline Ameliorates Neuro- and Genotoxicity Induced by Acute Malathion Intoxication in Rats
Galal, A., Salem, L., Hassanane, M., Nada, S., Abdel-Salam, O. (2019). Citicoline Ameliorates Neuro- and Genotoxicity Induced by Acute Malathion Intoxication in Rats. EKB Journal Management System, 5(2), 246-261. doi: 10.21608/jbaar.2019.146800
Asmaa F. Galal; Lamiaa M. Salem; Mahrousa M. Hassanane; Somaia A. Nada; Omar M. E. Abdel-Salam. "Citicoline Ameliorates Neuro- and Genotoxicity Induced by Acute Malathion Intoxication in Rats". EKB Journal Management System, 5, 2, 2019, 246-261. doi: 10.21608/jbaar.2019.146800
Galal, A., Salem, L., Hassanane, M., Nada, S., Abdel-Salam, O. (2019). 'Citicoline Ameliorates Neuro- and Genotoxicity Induced by Acute Malathion Intoxication in Rats', EKB Journal Management System, 5(2), pp. 246-261. doi: 10.21608/jbaar.2019.146800
Galal, A., Salem, L., Hassanane, M., Nada, S., Abdel-Salam, O. Citicoline Ameliorates Neuro- and Genotoxicity Induced by Acute Malathion Intoxication in Rats. EKB Journal Management System, 2019; 5(2): 246-261. doi: 10.21608/jbaar.2019.146800

Citicoline Ameliorates Neuro- and Genotoxicity Induced by Acute Malathion Intoxication in Rats

Journal of Bioscience and Applied Research
Article 10, Volume 5, Issue 2, June 2019, Page 246-261  XML PDF (692.72 K)
Document Type: Original Article
DOI: 10.21608/jbaar.2019.146800
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Authors
Asmaa F. Galal email 1; Lamiaa M. Salem2; Mahrousa M. Hassanane2; Somaia A. Nada3; Omar M. E. Abdel-Salam1
1Narcotics, Ergogrnics and Poisons Department, National Research Centre, Giza, Egypt
2Department of Cell Biology, National Research Centre, Giza, Egypt
3Pharmacology Department, National Research Centre, Giza, Egypt
Abstract
Objective: In the present study, we explored the therapeutic potential of citicoline in preventing malathion induced neurotoxicity and genotoxicity. Citicoline has been shown to act as a neuroprotective in experimental animal models of ischemia and other types of brain injuries. Methods: Acute malathion intoxication was induced by intraperitoneal injection of malathion (150 mg/Kg, once per day) for two successive days. Citicoline was co-administered in three doses (100, 200, 300 mg/kg, p.o.). Serum Butyrylcholine esterase (BChE) and Paraoxonase-1 (PON-1) were assessed as exposure biomarkers. Oxidative stress biomarkers were assessed in the different brain regions (cortex, striatum, and subcortex) in addition to TNF-α. Genotoxicity was tested by chromosomal aberrations, mitotic index, DNA fragmentation, and micronucleus tests. Results: Malathion administration resulted in marked suppression of serum BChE and PON-1 activities. Also, the exposure to the pesticide led to elevated malondialdehyde (MDA), nitric oxide (NO), and decreased reduced glutathione (GSH) levels in investigated brain regions in addition to elevated striatal tumor necrosis factor-α (TNF-α) level. Malathion also caused profound structural chromosomal aberrations, increased liver DNA fragmentation, and mitotic index. These effects were alleviated with the administration of citicoline dose-dependently. Conclusion: Our data indicate that citicoline can protect against malathion neurotoxic and genotoxic potential, possibly through antioxidant, anti-inflammatory activities, and restoring energy stores
Keywords
Malathion; Citicoline; Cytogenic damage; Oxidative stress; Inflammatory cytokines
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