BIOCHEMICAL AND MOLECULAR CHARACTERIZATION OF SOME YEAST ISOLATES | ||||
| Arab Universities Journal of Agricultural Sciences | ||||
| Article 8, Volume 15, Issue 2, September 2007, Page 315-324 PDF (306.51 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ajs.2007.14843 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| A. Abulhamd* 1; M M Mokhtar* 2; Rasha Mohamed Farrag,2 | ||||
| 1Department of Botany and Microbiology, Faculty of Science, Al-Azhar University, Cairo, Egypt | ||||
| 2The Regional Center for Mycology and Biotechnology, Al-Azhar University, Cairo, Egypt | ||||
| Abstract | ||||
| Sixteen yeast isolates from various sources in-cluding molasses, foodstuffs, concentrated juices and soils were identified using simplified API 20 C AUX yeast identification system and random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR). The isolates were found to belong to 11 species; Candida famata, C. glabra-ta, C. krusei / inconspicua, C. norvegensis, C. col-liculosa, Cryptococcus humicolus, Rhodotorula mucilaginosa, Kloeckera spp., Geotrichum capita-tum and Saccharomyces cerevisiae. API and RAPD-PCR proved to be useful and convenient taxonomic tools for rapid identification at the spe-cies level, proving that at least two techniques are needed for precise identification. RAPD-PCR pro-cedure is faster than traditional characterization techniques; the identification of a single colony was typically achieved in less than 10 hr. | ||||
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