A contribution on Coliforms causing mastitis in cows with reference to serotypes and virulence factors of E. coli isolates إسهام ميکروبات الکوليفورم في إحداث التهاب الضرع في الأبقار، مع الإشارة إلي التصنيف السيرولوجي وتحديد عوامل الضراوة لعترات الميکروب القولوني | ||||
Assiut University Bulletin for Environmental Researches | ||||
Article 7, Volume 17.1, Issue 17.1, March 2014, Page 85-95 PDF (532.57 K) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/auber.2014.148434 | ||||
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Abstract | ||||
ABSTRACT Escherichia coli (E. coli) is the predominant coliform species causing intramammary infections. Where in the present study, E. coli isolates were 18 strains (17.82%) followed by Enterobacter aerogenes 3 strains (2.97%) and Klebsiella pneumoniae one strain (0.99%) from 101 clinical mastitic milk samples of cows. Eighteen E. coli isolates were serotyped to nine different serogroups; O111:H4 (3), O127:H6 (3), O26 (2), O126 (2), O119:H6 (1), O114:H21 (1), O55:H7 (1), O44:H18 (1), O124 (1) and (3) untyped. Virulence tests were performed on the 18 isolated E. coli, it was found that 15 isolates (83.3%) were serum resistant, 13 isolates (72.2%) had Congo Red binding activity, 6 isolates (33.3%) were invasive and one isolate (5.6%) had haemolytic activity. PCR was applied to detect the presence of Shiga like toxin producing E. coli (stx1 and stx2 genes) on the nine different strains (one strain for each serogroup), where stx1 and stx2 were found in 8 (88.9%) and 4 (44.4%) of the nine examined strains, respectively. While stx1 and stx2 genes were found together in 3 strains (33.3%). Conclusions: E. coli isolates usually posses one or more virulence factors that may help in establishment at the infection site and subsequently causing clinical bovine mastitis. الملخص العربي : يعتبر الميکروب القولوني الأکثر شيوعاً في مجموعة ميکروبات الکوليفورم المسببة لالتهاب الضرع في الأبقار. حيث تم عزل ١٨ عترة (١٧،٨٢%) من الميکروب القولوني يليها الأنتيروباکتر أيروجينز ٣ عترات (٢،٩٧%) ثم ميکروب الکيلبيسيلا نيموني عترة واحدة (٠،٩٩%) من ١٠١ عينة لبن لأبقار إصابة بالتهاب الضرع الإکلينيکي. تم أجراء الفحص السيرلوجي لعترات الميکروب القولوني حيث تبين أنها تنتمي إلي تسع O111:H4(3), O127:H6(3), O26(2), O126 (2), O119:H6 (1), O114:H21 (1), هي مجموعات الضراوة اختبارات بإجراء .تصنيفها يتم لم عترات وثلاث O55:H7 (1), O44:H18 (1) and O124 (1) لعترات الميکروب القولوني المعزولة (١٨ عترة), وجد ١٥ عترة (٨٣،٣%) مقاومة للمصل و١٣ عترة (٧٢،٢%) لديها القدرة علي الارتباط بصبغة الکونغو الأحمر و6 عترات (٣٣،٣%) لديها القدرة علي أحداث التهاب ملتحمة العين في خنازير غينيا وعترة واحدة (٥،٦%) لديها المقدرة علي تحليل الدم. وباستخدام تفاعل البلمرة المتسلسل علي تسع عترات مختلفة للميکروب القولوني (عترة واحدة لکل مجموعة), وجد ٨ عترات (٨٨،٩%) تحتوي علي الجين ٤ stx1 و عترات (٤٤،٤ ت%) حتوي علي الجين stx2. کما أسفرت النتائج عن ثلاث عترات (٣٣،٣%) تحتوي علي الجينين stx1 and stx2 معا. الخلاصــة: عترات الميکروب القولوني المعزولة لديها واحد أو أکثر من عوامل الضراوة التي تمکنها من إحداث التهاب الضرع الإکلينيکي. | ||||
Keywords | ||||
Keywords: coliforms; E. coli; serotypes; virulence factors; stx1 and stx 2 | ||||
References | ||||
REFERENCES Amira, El-Sayed Lamey; Ammar, A. M.; Zaki, E. R.; Norhan, K.; Badea S. M. and Refai, M. K. (2013): Virulence factors of Escherichia coli isolated from recurrent cases of clinical and subclinical mastitis in buffaloes. International J. of Microbiological Research, 4 (1): 86- 94. Berkhoff, H. A. and Vinal, C. A. (1986): Congo red medium to distinguish between invasive and noninvasive Escherichia coli pathogenic for poultry. Avian Diseases, 30(1): 117-121. Beutin, L.; Krause, G.; Zimmermann, S.; Kaulfuss, S. and Gleier, K. (2004): Characterization of Shiga toxinproducing Escherichia coli strains isolated from human patients in Germany over a 3-year period. J. Clin. Microbiol. 42:1099-1108. Beutin, L.; Montenegro, M. A.; Orskov, I.; Orskov, F.; Prada, J.; Zimmermann, S. and Stephan, R. (1989): Close association of verotoxin (Shiga like toxin) production with enterohaemolysin production in strains of E. coli. J. Clin. Microbiol. 27(11):2559-2564. Blum, S. E. and Leitner, G. (2013): Genotyping and virulence factors assessment of bovine mastitis Escherichia coli. Vet. Microbiol., 163(3-4):305-312. Bradley, A.J. and Green, M.J. (2001): Aetiology of clinical mastitis in six Somerest dairy herds. Vet. Rec., 148(22):683- 686. Breen, J. E.; Green, M. J. and Bradley, A. J. (2009): Quarter and cow risk factors associated with the occurrence of clinical mastitis in dairy cows in the United Kingdom. J Dairy Sci., 92(6): 2551– 2561. Burvenich, C.; Van Merris, V.; Mehrzad, J.; Diez-Fraile, A. and Duchateau, L. (2003): Severity of E. coli mastitis is mainly determined by cow factors. Vet. Res., 34(5):521- 564. Dalia, M. Mohsen and Amany, N. Dapgh (2007): Detection of gene sequence of E. coli toxins isolated from mastitic buffalo and cows milk. Vet. Med. J. Giza, 55(2): 451-467. DaRocha, A.C.; DaSilva, A.B.; DeBrito, A.B.; Moraes, H. L.; Pontes, A.P.; Ce, M.C.; DoNascimento, V.P. and Salle, C.T. (2002): Virulence factors of avian pathogenic E. coli isolated from broilers from the South of Brazil. Avian Dis., 46(3):749-753. El-Khodery, S. A. and Osman, S. A. (2008): Acute coliform mastitis in buffaloes (Bubalus bubalis): Clinical findings and treatment outcomes. Trop. Animal Health and Production, 40(2): 93-99. El-Mahrouki, A. M.; Nevine, M. Sobhy and Aggour, M. G. (2006): Detection of coliform mastitis in cattle with special reference to molecular characterization of enterotoxigenic E. coli using polymerase chain reaction (PCR). J. Egypt. Vet. Med. Ass., 66(1): 47-58. Ericsson, U. H.; Lindberg, A.; Persson, W. K.; Ekman, T.; Artursson, K.;Nilsson-Ost M. and Bengtsson, B. (2009): Microbial aetiology of acute clinical mastitis and agent-specific risk factors. Vet. Microbiol., 137(1- 2):90-97. Fagan, P.; Hornitzky, M.; Bettelheim, K. and Djordjevic, S. (1999): Detection of Shiga-like toxin (stx1 and stx2), intimin (eaeA), and enterohemorrhagic E. coli (EHEC) hemolysin (EHEC hlyA) genes in animal feces by multiplex PCR. Appl. Environ. Microbiol. 65:868-872. Fernandes, JB. C.; Zanardo, L. G.; Galvão, N.N.; Carvalho, I. A.; Nero, L. A. and Moreira, M.A. S. (2011): Escherichia coli from clinical mastitis: serotypes and virulence factors. J. of Vet. Diagnostic Investigation, 23(6) 1146–1152. Gad EL-Said, W. A.; El-Jakee, J. K.; Xandel, M. M. and Mona, A. ElShabrawy (2005): Presence of E. coli O157:H7 in raw milk and meat samples. J. Egypt Vet. Med. Ass., 65(3): 341- 350. Galal, H. M.; Hakim, A. S. and Sohad, M. D. (2013): Phenotypic and virulence genes screening of Escherichia coli strains isolated from different sources in delta Egypt. Life Science Journal, 10(2):352-361. Güler, L. and Gündüz, K. (2007): Virulence properties of Escherichia coli isolated from clinical bovine mastitis. Turk. J. Vet. Anim. Sci., 31(5): 361-366. Gyles, C. L. (1993): Escherichia coli. In: Gyles, C. L., Thoen, C.O. (Eds.). Pathogenesis of bacterial infections in animals. Iowa State University Press, Ames, Iowa, pp. 164-187. Hogan, J. S and Smith, K. L. (2003): Coliform mastitis. Vet. Res., 34:507-519. John, M.; Jacques, M. and Lariviere, S. (1989): Pathogenicity of E.coli O115:K V165 strains isolated from pigs with diarrohea. Am. J. Vet. Res., 50(1): 1029- 1036. Kaipainen, T.; Pohjanvitra, T.; Sphigel, N. Y.; Shwimmer, A.; Pyolara, S. and Pelkonen, S. (2002): Virulence factors of E. coli isolated from bovine clinical mastitis. Vet. Microbiol., 85(1):37-46. Kalmus, P.; Aasmäe, B.; Kärssin, A.; Orro, T. and Kask, K. (2011): Udder pathogens and their resistance to antimicrobial agents in dairy cows in Estonia. Acta Vet Scand. 53: 4. Kaper, J. B.; Nataro, J. P. and Mobley, H. L. (2004): Pathogenic E. coli. Nat. Rev. Microbiol., 2:123–140. Kok, T.; Worswich, D. and Gowans, E. (1996): Some serological techniques for microbial and viral infections. In Practical Medical Microbiology (Collee, J.; Fraser, A.; Marmion, B. and Simmons, A., eds.) 14th ed., Edinburgh, Churchill Livingstone, UK. Koneman, E. W.; Allen, S. D.; Dowell V. R. and Sommer H. M. (1983): The Enterobacteriaceae, in: Color Atlas and Textbook of Diagnostic Microbiology, J.B. Lippincott Company, New York, USA, pp. 57– 124. Lehtolainen, T. (2004): E. coli mastitis: Bacterial factors and host response, Ph. D. thesis Department of clinical veterinary sciences, Faculty of veterinary Medicine, Helsinki, University, Finland. Lira, W. M.; Macedo, C. and Marin, J. M. (2004): The incidence of Shiga toxinproducing Escherichia coli in cattle with mastitis in Brazil. J.of Applied Microbiology, 9: 861–866. Longo, F.; Salat, O. and Gool, V.F. (2001): Incidence of clinical mastitis in French dairy herds: epidemiological data and economic costs. Folia Veterinaria, 45: 45-46. McKillip, J. and Drake, M. (2000): Molecular beacon polymerase chain reaction detection of Escherichia coli O157:H7 in milk. J. Food Protect. 63: 855-859. Momtaz, H.; Dehkordi, F. S.; Taktaz, T.; Rezvani, A. and Yarali, S. (2012): Shiga toxin-producing Escherichia coli isolated from bovine mastitic milk: Serogroups, virulence factors, and antibiotic resistance properties. The Scientific World Journal, Article ID 618709, 9 pages. Mosherf, B. E. (2004): Studies on microbial causes of mastitis in buffaloes, Ph. D. thesis (Microbiology), Fac. Vet. Med. Cairo Univ. Moussa, I. M.; Mostafa, M. and Mohamed, K. F. (2006): Determination of phylogenetic relationships among E. coli isolates recovered from bovine fecal and milk samples. J. Egypt. Vet. Med. Ass., 66: 7-25. Murinda, S.E.; Nquyen, L.T.; Landers, T.L.; Draughon, F.A.; Mathew, A.G.; Hogan, J.S.; Smith, K.L.; Hancock, D.D. and Oliver, S.P. (2004): Comparison of Escherichia coli isolates from humans, food and farm and companion animals for presence of Shiga toxin-producing E. coli virulence markers. Foodborne Pathogens and Disease, 1(3): 178-184. Oliver, S.P. (1988): Frequency of isolation of environmental mastitis-causing pathogens and incidence of new intramammary infection during the non-lactating period. American Journal of Veterinary Research, 49(11): 1789-1793 . Panigrahy, B. and Yushen, L. (1990): Differentiation of pathogenic and non pathogenic Escherichia coli isolated from poultry. Avian Dis., 34: 941-943. Pass, M., Odedra, R. and Batt, R. (2000): Multiplex PCR for identification of Escherichia coli virulence genes. J. Clin. Microbiol. 38:2001-2004. Quesnell, R. R.; Klaessig, S.; Watts, J. L. and Schukken, Y. H. (2012): Bovine intramammary Escherichia coli challenge infections in late gestation demonstrate a dominant anti-inflammatory immunological response. J. Dairy Sci., 95(1):117-126. Quinn, P. J.; Carter, M. E.; Markey, B. and Carter, G. R. (2004): Clinical veterinary microbiology. 6th ed., Mosby, Edinburgh, London, New York, Philadelphia, St. Louis, Sydney, Toronto. Rangel, P. and Marin, J. M. (2009): Analysis of Escherichia coli isolated from bovine mastitic milk. Pesq. Vet. Bras. 29(5):363-368. Salman, A. M. (1999): Serological and bacteriological studies on Escherichia coli in chicken. Ph. D. Thesis (Microbiology), Fac. Vet. Med., Cairo Univ. Schukken, Y.H,; Bennett, G.J.; Zurakowski, M.J.; Sharkey, H.L.; Rauch, B.J.; Thomas, M.J.; Ceglowski, B.; Saltman, R.L.; Belomestnykh, N. and Zadoks R.N. (2011): Randomized clinical trial to evaluate the efficacy of a 5-day ceftiofur hydrochloride intramammary treatment on non severe gram-negative clinical mastitis. J. Dairy Sci., 94(12):6203-6215. Sereny, B. (1955): Experimental shigella keratoconjunctivitis. Acta Microbiol. Acad. Sci. Hung., 2: 293-296. Sharma, K. K.; Soni, S. S. and Meharchandani, S. (2006): Congo red dye test as an indicator test for detection of invasive bovine Escherichia coli – short communication. Veterinarski Arhiv, 76(4):363-366. Taylor, P. w. (1983): Bacteriocidal and bacteriolytic activity of serum against Gram negative bacteria. Microbial. Rev., 47: 46-83. Timmis, K. N. (1979): Plasmid gene that specifies resistance to the bactericidal activity of serum. In Plasmids of medical, environmental and commercial importance. Edited by K. N. Timmis & Puhler, A. (Elsevier, North Holland Biomedical Press, New York, 145-153. Wenz, J. R.; Barrington, G. M.; Garry, F. B.; Ellis, R. P. and Magnuson, R. J. (2006): E. coli isolates, serotypes, genotypes and virulence genes and clinical coliform mastitis severity. Journal of Dairy Science, 89(9): 3408-3412. Zaki, E.R.; Riad, E. M. and Sobhy, N. M. (2004): Correlation between E. coli serotypes isolated from buffalo mastitic milk with different virulence patterns. J. Egypt Vet. Med. Assoc., 64: 53-63. Zschock, M.; Hamann, H. P.; Kloppert, B. and Wolter, W. (2000): Shiga-toxin producing E.coli in faeces of healthy dairy cows, sheep and goats: prevalence and virulence properties. Lett. Appl. Microbiol. 31:203-208. | ||||
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