MORPHOLOGICAL AND MOLECULAR CHARACTERIZATION OF ECTOMYCORRHIZAL FUNGI ISOLATED FROM EGYPTIAN ENVIRONMENT | ||||
Arab Universities Journal of Agricultural Sciences | ||||
Article 9, Volume 15, Issue 2, September 2007, Page 325-335 PDF (362.26 K) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/ajs.2007.14854 | ||||
View on SCiNiTO | ||||
Authors | ||||
Mona Zayed* 1; Sh Selim2; Wedad E Eweeda3; M K Ali4; A Hazem5 | ||||
1Microbiology Dept.. Fac. Agric., Ain Shams Univ., Shobra El-Kheima, Cairo 2-Unit of Biofertiliz., Fac. Agric., Ain Shams Univ., Shobra El-Kheima, Cairo | ||||
21-Microbiology Dept.. Fac. Agric., Ain Shams Univ., Shobra El-Kheima, Cairo 2-Unit of Biofertiliz., Fac. Agric., Ain Shams Univ., Shobra El-Kheima, Cairo | ||||
31. Microbiology Dept.. Fac. Agric., Ain Shams Univ., Shobra El-Kheima, Cairo 2. Unit of Biofertiliz., Fac. Agric., Ain Shams Univ., Shobra El-Kheima, Cairo | ||||
4Plant Pathology Dept., Fac. Agric., Ain Shams Univ., Shobra El-Kheima, Cairo | ||||
5Microbiology Dept.. Fac. Agric., Ain Shams Univ., Shobra El-Kheima, Cairo | ||||
Abstract | ||||
In a trial to isolate and identify ectomycorrhi-zal fungi for the first time in Egypt 13 sporocarpes associated with rang of plants grown on Egyptian soils were collected, to be used for this purpose. Fungal isolates were obtained from collected spo-rocarpes and tested for ectomycorrhiza formation with Bauhinia sp. and Pinus seedlings. The paper sandwich technique was used for simultaneous inoculation of root apices Bauhinia to produce synchronously developing ectomycorrhizas. Typi-cal ectomycorrhizal roots were obtained within 14 days after inoculation with the collected strains. The isolates tested for ectomycorrhiza formation with Pinus sp. to ensure their identity by for-mation of distinct root characteristics on this host. Root colonization levels varied markedly among the tested fungal strains with respect to growth enhancement and NPK uptake of Pinus shoots and roots. | ||||
Keywords | ||||
isolation; Sporocarpes; identification; Ectomycorrhizae; Growth parameters of Pinus | ||||
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