Molecular Characterization, Full Length Isolation and Phylogenetic Analysis of C-type Lectin Gene from Bacterial-challenged Cotton Leafworm, Spodoptera littoralis | ||||
| Egyptian Academic Journal of Biological Sciences. A, Entomology | ||||
| Article 3, Volume 5, Issue 1, April 2012, Page 31-44 PDF (1.04 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/eajbsa.2012.14905 | ||||
 View on SCiNiTO | ||||
| Author | ||||
| AlaaEddeen M. Seufi | ||||
| Entomology Department, Faculty of Science, Cairo University, Giza, Egypt | ||||
| Abstract | ||||
| Experiments were designed to investigate the molecular immune response of Spodoptera littoralis larvae against bacterial infection. In addition, sequence and phylogenetic analyses of the involved gene were studied. Using differential display technique, a partial insect lectin gene (SpliLec) was isolated from bacterial-challenged S. littoralis haemolymph. Five differentially displayed bands were sequenced. Sequence results revealed that a fragment of 640 bp was amplified within the open reading frame (orf) of a lectin gene. This fragment contained the complete 3` end with a poly(A) tail, but it lacks start codon, AUG at its 5` end. Using RACE PCR reaction, 5` end was extended and a final reaction was performed to obtain the full length of the SpliLec. Sequence analyses of the data revealed that SpliLec consists of a single orf encoding a deduced polypeptide consisting of a 18-residue signal peptide and a 291- residue mature peptide. SpliLec sequence contained two CRDs: short form CRD1 and long form CRD2 stabilized by two and three highly conserved disulfide bonds, respectively. SpliLec shares homology with some dipteran lectins suggesting possible common ancestor. These results suggested an important role of the SpliLec gene in cell adhesion and non-self recognition. It may cooperate with other AMPs in clearance of invaders of Spodoptera littoralis. | ||||
| Keywords | ||||
| Immune system; Spodoptera; C-type lectin; CRD; PAMP | ||||
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