EFFECT OF DIETARY ZINC, COPPER AND IRON LEVELS ON SUPEROXIDE DISMUTASE, CATALASE AND GLUTATHIONE-S- TRANSFERASE ACTIVITY
El-Dakak, A., Ahmed, M., El-Nahal, D. (2007). EFFECT OF DIETARY ZINC, COPPER AND IRON LEVELS ON SUPEROXIDE DISMUTASE, CATALASE AND GLUTATHIONE-S- TRANSFERASE ACTIVITY. EKB Journal Management System, 15(2), 371-382. doi: 10.21608/ajs.2007.14927
Abeer M.N.H. El-Dakak; Mona H.M Ahmed; Dalia M.M El-Nahal. "EFFECT OF DIETARY ZINC, COPPER AND IRON LEVELS ON SUPEROXIDE DISMUTASE, CATALASE AND GLUTATHIONE-S- TRANSFERASE ACTIVITY". EKB Journal Management System, 15, 2, 2007, 371-382. doi: 10.21608/ajs.2007.14927
El-Dakak, A., Ahmed, M., El-Nahal, D. (2007). 'EFFECT OF DIETARY ZINC, COPPER AND IRON LEVELS ON SUPEROXIDE DISMUTASE, CATALASE AND GLUTATHIONE-S- TRANSFERASE ACTIVITY', EKB Journal Management System, 15(2), pp. 371-382. doi: 10.21608/ajs.2007.14927
El-Dakak, A., Ahmed, M., El-Nahal, D. EFFECT OF DIETARY ZINC, COPPER AND IRON LEVELS ON SUPEROXIDE DISMUTASE, CATALASE AND GLUTATHIONE-S- TRANSFERASE ACTIVITY. EKB Journal Management System, 2007; 15(2): 371-382. doi: 10.21608/ajs.2007.14927

EFFECT OF DIETARY ZINC, COPPER AND IRON LEVELS ON SUPEROXIDE DISMUTASE, CATALASE AND GLUTATHIONE-S- TRANSFERASE ACTIVITY

Arab Universities Journal of Agricultural Sciences
Article 13, Volume 15, Issue 2, September 2007, Page 371-382  XML PDF (442.48 K)
Document Type: Original Article
DOI: 10.21608/ajs.2007.14927
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Authors
Abeer M.N.H. El-Dakak* ; Mona H.M Ahmed; Dalia M.M El-Nahal
Special Food and Nutrition Dept., Food Technology Research Institute, Agric. Res. Center, Giza, Egypt
Abstract
The interactions between dietary zinc, copper and iron and their effects on antioxidant enzymes activity [superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase (GST)] were examined. Fifty-four rats weighting approximately between 80-100 grams were divided into nine groups (E1- E9). Each group containing six rats was fed on basal diet with varying amount of Zn (2.5, 35 or 175 mg Zn/kg diet), Cu (1.2, 6.3, or 25 mg Cu/kg diet) and Fe (10, 50 or 230 mg Fe/kg diet). The second group (E2) was fed a diet defi-cient in all studied elements (2.5mg Zn, 1.2mg Cu, and 10mg Fe per 1 kg diet). The results of E2 showed a decrease in SOD and CAT activity and increase GST in plasma erythrocytes and tissues. Moreover, plasma thiobarbituric acid reactive sub-stances (TBARs) and hydrogen peroxide produc-tion in E2 were found to be higher than those in other groups. The high activity of lactic acid de-hydrogenase (LDH) in the plasma indicated that cell membrane damage was occurred. This dam-age was accompanied by elevation of lipid peroxi-dation and in the same time by reduction of SOD and CAT activity. Supplementation of basal diet with the different concentrations of studied miner-als led to improve the activity of antioxidants en-zymes (SOD and CAT) and decrease in TBARs and LDH activity.
Keywords
zinc; copper; iron; Superoxide dis-mutase; catalase; glutathione-S-transferase
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