INFORMALLY RAW MILK AND KAREISH CHEESE INVESTIGATION ON THE OCCURRENCE OF TOXIGENIC ESCHERICHIA COLI IN QENA CITY, EGYPT WITH EMPHASIS ON MOLECULAR CHARACTERIZATION تعييـن حدوث بکتيريا القولون المعدي المفرزة للسموم في الألبان الطازجة والجبن القريش في مدينة قنا- مصر مع التشخيص الجزيئي | ||||
| Assiut University Bulletin for Environmental Researches | ||||
| Article 4, Volume 11.2, Issue 11.2, October 2009, Page 35-41 PDF (459.14 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/auber.2008.149623 | ||||
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| Abstract | ||||
| ABSTRACT: The informally raw milk and Kareish cheese sold in Qena city (Upper Egypt) were analyzed to determine the presence of toxigenic E.coli. The isolates were screened for the presence of verotoxigenic E.coli (VTEC) and enterotoxigenic E.coli (ETEC) by Polymerase Chain Reaction (PCR). Bio-resistance to antimicrobial agents was evaluated by the disk diffusion method. E coli were recovered from 38 (76%) of raw milk and 11 (47.8%) of Kareish cheese samples. Three (6.1%) of the E.coli isolates were VTEC and none of them hat eaeA gene encoded a pathogenicity island typical of E.coli O157:H7 (EHEC). PCR of enterotoxins showed that only one isolate carried LT enterotoxins of ETEC. Bio-resistance was frequently observed to nalidixic acid (42.9%), ampicillin (32.7%), tetracycline (22.4%), trimethoprim-sulfamethoxazol (14.3%), ciprofloxacin (4.1%) and cefoxtin (2.0%). Results suggested a possibility of potential public health threat of E.coli originating from raw milk sources. تم إجراء دراسة بکتيريولوجية على بعض الألبان والجبن القريش التى تباع في أسواق مدينة قنا – مصر وذلک لتعيين مدى وجود بکتيريا القولون المعدي المفرزة للسموم. تم إجراء تعريف للعزلات المختارة إلى مستوي النوع، وکذلک تعيين الجينات المسئولة عن الضراوة والأمراضية باستخدام تفاعل البلمرة. تم تقييم مقاومة تلک العزلات لمختلف المضادات الميکروبية.أشارت النتائج بشکل عام إلى عزل نسبة عالية من ميکروب القولون المعدي، مما يدل على انخفاض الجودة. أظهرت نتائج تفاعل البلمرة الکشف عن وجود بعض الجينات المسئولة عن إحداث تلک العزلات لأمراض، وکذلک مقاومة بعض العزلات لفعل المضادات الميکروبية المختلفة. أوصت الدراسة باحتمالية خطورة استهلاک تلک الألبان الطازجة ومنتجاتها (الجبن القريش) على الصحة العامة. | ||||
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