Gamma Dose-Dependence of Calcium Phosphate Growth on DFDBA Subsequent to SBF Immersion | ||||
Egyptian Journal of Solids | ||||
Article 12, Volume 27, Issue 1, 2004, Page 135-147 PDF (1.35 MB) | ||||
DOI: 10.21608/ejs.2004.149840 | ||||
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Abstract | ||||
The simulated body fluid (SBF) induced epitaxial calcium phosphate growth on demineralized freeze dried bone allograft (DFDBA) xenogeneic bone matrices as a function of 60Co-gamma sterilization with single shot technique of 15,25, 30 and 40 KGy. Surface morphology by Scanning electron microscope (SEM) supplemented by EDAX as well as FT-IR analyses were conducted for the solids pre-, post-irradiation and post-immersion in SBF up to 72 hours. The ability of the tissues to form bone was predicted through Ca and iP ions measurements in SBF as a function of immersing times at each applied gammadose. The epitaxial growth of octacalcium phosphate (OCP) with rose like morphology characteristically defined 15 KGy as the most effective gamma dose on inducing biolayer formation. FT-IR analyses correlated precisely with the degree of denaturation of protein with 30 KGy and the structure reverted to a state similar to pre-irradiation with 40 KGy. | ||||
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