STUDYING EFFECT OF pH ON THE ANTIMYCOTIC PERFORMANCE OF SOME DISINFECTANTS BY USING QUANTITATIVE SUSPENSION TEST استخدام اختبار المعلق الکمي في دراسة تأثير الأس الهيدروجيني علي کفاءه المطهرات | ||||
| Assiut University Bulletin for Environmental Researches | ||||
| Article 6, Volume 7.1, Issue 7.1, March 2004, Page 45-54 PDF (649.55 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/auber.2004.150596 | ||||
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| Abstract | ||||
| ABSTRACT : The antimycotic effect of some disinfectants was comparatively studied under different pH gradients. Reference strains of C.albicans, A.niger and T.mentagrophytes were used in the current study. Quantitative suspension test was used with an initial inoculum of 1.0x107 CFU/mL from each organism. After different exposure time (1; 2; 5; 10; 15; 30 and 60 min), 1.0 mL was taken and added to 9.0 mL of the neutralizer to give the required concentration and left for 10 min before spreading of 0.1mL on Sabouraud Dextrose agar medium. The CFU/mL was recorded for each time point and the reduction of the viable count was recorded as log10 of the count. The obtained results revealed that glutaraldehyde, formaldehyde, phenols and quaternary ammonium compounds are highly effective at alkaline pH. The acid pH gradient of these compounds either fails to give the required result or needs a longer time of exposure. On the other hand, sod. hypochlorite and standard phenol were showed high antimycotic efficiencies under acidic pH than alkaline ones. Using the alkalinizing and/or acidifying agents observed no direct toxic effect on the organism. This indicates that the pH may modify the disinfectant performance, which should be put in our mind during disinfection. في هذا البحث تم دراسة تأثير عدد من المطهرات واسعة الانتشار في الحقل البيطري علي ميکروبات کانديدا البيکانز والأسبرجيلس نيجر وکذلک الترايکوفايتون منتاجروفايتس لمعرفة اقل ترکيز کافي لقتل هذه الميکروبات عند أوقات مختلفة تراوحت من دقيقه واحدة وحتى 60 دقيقه0 تم تغيير الأس الهيدروجيني لهذه المرکبات للقاعدي باستخدام 0.3% بيکربونات الصوديوم وکذلک للحمضي باستخدام 0.01% حمض الهيدروکلوريک0 ثم اختبرت هذه المرکبات عند نفس الترکيز علي نفس الميکروبات السابق ذکرها0 قورنت النتائج عند درجات الأسس الهيدروجينية المختلفة. أثبتت النتائج أن الأس الهدروجيني يلعب دورا هاما في کفاءه المطهرات التي استخدمت في هذا البحث0 في هذا الإطار ثبت أن مرکبات الجلوترالديهات ومرکبات الأمونيا الرباعية ومرکب أل Tek-Trol کانت أعلي کفاءه عند الأس الهيدروجيني القاعدي وأن تغيير الأس الهيدروجيني لهذه المرکبات إلى الحمضي فشل في قتل الميکروبات کما أن بعض المرکبات قد احتاج وقت أطول للحصول علي نفس النتائج التي تم الحصول عليها من هذه المرکبات عندما تکون قاعدية، ومن ناحية أخري فان مرکب هيبوکلوريدات الصوديوم کانت أعلي کفاءه عند شقها الحامضي. بالرغم من أن الشق القاعدي لمرکبات الفورمالدهيد والفينول کانت أعلي من الشق الحمضي لها لکنها کانت اعلي کفاءه عند الأس الهيدروجيني المتعادل، من هذه النتائج ينصح بوضع الأس الهيدروجيني في الاعتبار عند استخدام هذه المطهرات وصولا لأعلي کفاءه تطهيريه0 | ||||
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