Role of Vitamin D in the Induction of Regulatory T Cells Producing Interleukin 10 in Children with Cow Milk Allergy | ||||
The Egyptian Journal of Hospital Medicine | ||||
Article 1, Volume 65, Issue 1, October 2016, Page 454-467 PDF (644.06 K) | ||||
Document Type: Original Article | ||||
DOI: 10.12816/0033753 | ||||
View on SCiNiTO | ||||
Authors | ||||
Randa Reda Mabrouk1; Hana Ahmed Amer1; Dina Ahmed Soliman2; Nesrine Aly Mohamed; Dalia Helmy El-Ghoneimy3; Sara Mohammad Atef | ||||
1Chemical Pathology Ain Shams University | ||||
2Clinical and Chemical Pathology Ain Shams University | ||||
3Pediatric, Faculty of Medicine – Ain Shams University | ||||
Abstract | ||||
Background: Various populations of regulatory T cells play a central role in the development of peripheral tolerance to allergens. Culturing of CD4+ T cells isolated from peripheral blood of allergic patients with vitamin D induces the generation of stable IL-10 producing CD4+CD25+ Treg cells suppressing the proliferation of T helper cells obtained from the same patients. The immune regulatory role of vitamin D in allergic patients has been controversial and obviously needs a more clarifying research work. Aim of the work: to determine the percentage of induced T regulatory cells producing interleukin 10 after stimulation of T regulatory cells with cow milk allergen in the presence of vitamin D in culture. This aims to further in-vitro study the immune regulatory role of vitamin D in cow milk allergic patients. Results: there is association between decreased level of vitamin D and milk-allergy, as serum level of 25(OH) D3 was insufficient in 16 (80 %) patients (10- 29.9 ng/ml) while 4 (20%) patients were sufficient (30-100 ng/ml). Addition of vitamin D, in culture, induces the production of CD4+ CD25hi Foxp3+ IL10+ . Treg cells within peripheral blood mononuclear cells (PMNCs) isolated from allergic children who had insufficient vitamin D, but not in allergic children who had normal level of vitamin D. Conclusion: this work provides further evidence for an important role of 1,25(OH)2D3 as an immune-modulatory molecule and suggests that supplementation of vitamin-D-deficient individuals, who are reported to have reduced numbers of circulating and Foxp3+ IL10+ Treg cells, may represent an attractive therapy for enhancing endogenous populations of Treg cells in allergy. | ||||
Keywords | ||||
Regulatory T cells; calcitriol; Food allergy | ||||
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