Egyptian Myrtus communis L. Essential oil Potential role as invitro Antioxidant, Cytotoxic and α-amylase Inhibitor
Ibrahim, F., Fouad, R., EL-Hallouty, S., Hendawy, S., Omer, E., Mohammed, R. (2021). Egyptian Myrtus communis L. Essential oil Potential role as invitro Antioxidant, Cytotoxic and α-amylase Inhibitor. EKB Journal Management System, 64(6), 3005-3017. doi: 10.21608/ejchem.2021.57354.3245
Faten M. Ibrahim; Rasha Fouad; Salwa EL-Hallouty; Saber F. Hendawy; Elsayed A. Omer; Reda S. Mohammed. "Egyptian Myrtus communis L. Essential oil Potential role as invitro Antioxidant, Cytotoxic and α-amylase Inhibitor". EKB Journal Management System, 64, 6, 2021, 3005-3017. doi: 10.21608/ejchem.2021.57354.3245
Ibrahim, F., Fouad, R., EL-Hallouty, S., Hendawy, S., Omer, E., Mohammed, R. (2021). 'Egyptian Myrtus communis L. Essential oil Potential role as invitro Antioxidant, Cytotoxic and α-amylase Inhibitor', EKB Journal Management System, 64(6), pp. 3005-3017. doi: 10.21608/ejchem.2021.57354.3245
Ibrahim, F., Fouad, R., EL-Hallouty, S., Hendawy, S., Omer, E., Mohammed, R. Egyptian Myrtus communis L. Essential oil Potential role as invitro Antioxidant, Cytotoxic and α-amylase Inhibitor. EKB Journal Management System, 2021; 64(6): 3005-3017. doi: 10.21608/ejchem.2021.57354.3245

Egyptian Myrtus communis L. Essential oil Potential role as invitro Antioxidant, Cytotoxic and α-amylase Inhibitor

Egyptian Journal of Chemistry
Article 31, Volume 64, Issue 6, June 2021, Page 3005-3017  XML PDF (534.72 K)
Document Type: Original Article
DOI: 10.21608/ejchem.2021.57354.3245
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Authors
Faten M. Ibrahimorcid 1; Rasha Fouadorcid 1; Salwa EL-Halloutyorcid 2; Saber F. Hendawy1; Elsayed A. Omerorcid 1; Reda S. Mohammed email orcid 2
1Medicinal and Aromatic Plants Research Department, National Research Centre; 33-Elbohouth St, (Former El-Tahrir St.), Dokki – Giza-Egypt. P.O.12622 ID: 60014618
2Department of Pharmacognosy, National Research Centre; 33-Elbohouth St, (Former El-Tahrir St.), Dokki – Giza-Egypt. P.O.12622, ID: 60014618
Abstract
Abstract
Myrtus communis L. (MC) family Myrtaceae, is cultivated in Egypt as an ornamental plant. The essential oil (EO) isolated from different parts of the plant reported for many pharmacological activities. Myrtus black and white (MB &MW) are two varieties of Myrtus diverse from each other in the color of berries; the EO was prepared from the fresh aerial parts of both varieties through hydrodistillation. The EO was screened for its free radicals scavenging activity by five different methods (DPPH, ABTS, NO, SOR, LP) and with Vit. C & BHT as reference standard. It was noticed that the scavenging activity of MB at all used conc. (125 to 1000 µg/mL) was more potent than MW. Screening of the cytotoxic activities of the EO of MB and MW on five cancer cell line (PC3, MCF-7, A549, HepG-2, HCT-116 ) & one normal cell line (Rpel-1) revealed that both EO of the two varieties had activity against PC3& MCF-7 with IC50; 4.7±0.15, 14.2±0.35, 45.2±0.67, 50.5±0.6 compared with Doxrubacin standard 24±0.26, 26.1±0.4, respectively. MB& MW oil are safe on Rpel-1 (normal cell line). The α-amylase enzyme inhibition activity of the oils was investigated using acarbose as reference inhibitor. Both MB& MW have activity more than the standard at 125μg/ml 36.24±0.146; 32.14±.188, 23.16±0.190 and also at the highest conc. (1000 µg/mL) 96.22±0.140, 89.48±.106, 88.81±0.690, respectively. Study of the chemical constituents of the EO by GC/MS analysis resulted in the identification of 25 compounds from both species constituting 99.21% & 99.96% of the total peak area of the identified compound, respectively. The major compounds are α–Pinene (49.33%, 31.10 %); 1,8 Cineole (9.97 %, 19.21 %); Myrtenyl acetate (30.7%, 40.80 %); α –Terpineol (2.18%, 1.73%) and D-Limonene (1.90% , 2.61%) for MB & MW, respectively.
Keywords
Myrtus communis; free radical scavenging activity; α- amylase inhibitor; cytotoxic activity; cell lines; GC/MS analysis
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