Tumour-associated macrophages (IL-23+CD14+ subset) levels during bladder cancer progression
El-Gedamy, M., Abolenein, D., Elkhayat, Z., Elsaid, A., Elnahrery, I. (2021). Tumour-associated macrophages (IL-23+CD14+ subset) levels during bladder cancer progression. EKB Journal Management System, 2(1), 11-18. doi: 10.21608/fsrt.2021.56346.1030
Mohammed Saad El-Gedamy; Dr. Hassan Abolenein; Zakaria Elkhayat; Afaf Elsaid; Islam M Elnahrery. "Tumour-associated macrophages (IL-23+CD14+ subset) levels during bladder cancer progression". EKB Journal Management System, 2, 1, 2021, 11-18. doi: 10.21608/fsrt.2021.56346.1030
El-Gedamy, M., Abolenein, D., Elkhayat, Z., Elsaid, A., Elnahrery, I. (2021). 'Tumour-associated macrophages (IL-23+CD14+ subset) levels during bladder cancer progression', EKB Journal Management System, 2(1), pp. 11-18. doi: 10.21608/fsrt.2021.56346.1030
El-Gedamy, M., Abolenein, D., Elkhayat, Z., Elsaid, A., Elnahrery, I. Tumour-associated macrophages (IL-23+CD14+ subset) levels during bladder cancer progression. EKB Journal Management System, 2021; 2(1): 11-18. doi: 10.21608/fsrt.2021.56346.1030

Tumour-associated macrophages (IL-23+CD14+ subset) levels during bladder cancer progression

Frontiers in Scientific Research and Technology
Article 3, Volume 2, Issue 1, 2021, Page 11-18  XML PDF (568.56 K)
Document Type: Original Article
DOI: 10.21608/fsrt.2021.56346.1030
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Authors
Mohammed Saad El-Gedamy1; Dr. Hassan Abolenein2; Zakaria Elkhayat3; Afaf Elsaid4; Islam M Elnahrery email orcid 5
1Chemistry(Biochemistry department), Suez University, Suez, Egypt
2Professor of urology, Mmansoura university
3Professor of Medical Biochemistry National Research Center, Giza, Egypt
4Genetics Unit, Children Hospital, Mansoura University, Egypt
5Suez University
Abstract
Background: Multiple studies have reported that tumour-associated-macrophages (TAMs), which are abundant in the tumour stroma, are related to poor prognosis in different tumours, such as pancreatic adenocarcinoma, gastric cancer, and bladder cancer (BC). They exert a pivotal role in modulating tumor-progression and adjusting response to immunotherapy. In this article, we strive to evaluate the significance of CD14-positive TAMs levels in relation to BC development. Methodology: Based on the immuno-phenotypic analysis, the counts of TAMs bearing CD14 and IL23-receptor (IL23R) fractions were determined in 15 healthy-controls and 26 BC-patients BC, using a panel of phycoerythrin (PE)-labelled monoclonal-antibodies. Concomitantly, serum-levels of IL23 and IL17 cytokines were identified by ELISA-technique. Results: Findings revealed a higher levels of CD14+ IL23+ TAMs in BC patients sera [P= 0.035] compared to controls, and this increment was positively associated with advancing in tumour-grade [P=0.001] and stage [P< 0.043]. In context of bladder carcinogenesis, concentration of the aforesaid TAM subpopulation correlates positively with increased levels of both IL23 [r = 0.66, P<0.01] and IL17 [r = 0.657, P<0.01]. Conclusions: The study suggested that CD14-positive TAMs, especially those harboring IL23R engage in inflammation-related tumourgenesis by fostering the up-regulation of the inflammatory IL23/17 immune axis, and further can be utilized as a prognostic marker for BC development and progression.
Keywords
Bladder cancer; tumor associated macrophages; interleukin 23 receptor; chronic inflammation
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