The effect of activated pure platelete rich plasma (P-PRP) on the proliferation of adipose -derived mesenchymal stem cells (AD-MSCs) as a substitute for fetal bovine serum (FBS)
El-Gamal, A., Kassab, M., Abd-Elmaksoud, A., Farag, A., Marie, H. (2019). The effect of activated pure platelete rich plasma (P-PRP) on the proliferation of adipose -derived mesenchymal stem cells (AD-MSCs) as a substitute for fetal bovine serum (FBS). EKB Journal Management System, 20(1), 35-40. doi: 10.21608/mvmj.2019.01.103
Aya El-Gamal; Mohammed Kassab; Ahmed Abd-Elmaksoud; Amany Farag; Hany Marie. "The effect of activated pure platelete rich plasma (P-PRP) on the proliferation of adipose -derived mesenchymal stem cells (AD-MSCs) as a substitute for fetal bovine serum (FBS)". EKB Journal Management System, 20, 1, 2019, 35-40. doi: 10.21608/mvmj.2019.01.103
El-Gamal, A., Kassab, M., Abd-Elmaksoud, A., Farag, A., Marie, H. (2019). 'The effect of activated pure platelete rich plasma (P-PRP) on the proliferation of adipose -derived mesenchymal stem cells (AD-MSCs) as a substitute for fetal bovine serum (FBS)', EKB Journal Management System, 20(1), pp. 35-40. doi: 10.21608/mvmj.2019.01.103
El-Gamal, A., Kassab, M., Abd-Elmaksoud, A., Farag, A., Marie, H. The effect of activated pure platelete rich plasma (P-PRP) on the proliferation of adipose -derived mesenchymal stem cells (AD-MSCs) as a substitute for fetal bovine serum (FBS). EKB Journal Management System, 2019; 20(1): 35-40. doi: 10.21608/mvmj.2019.01.103

The effect of activated pure platelete rich plasma (P-PRP) on the proliferation of adipose -derived mesenchymal stem cells (AD-MSCs) as a substitute for fetal bovine serum (FBS)

Mansoura Veterinary Medical Journal
Article 10, Volume 20, Issue 1, March 2019, Page 35-40  XML PDF (598.42 K)
Document Type: Original Articles
DOI: 10.21608/mvmj.2019.01.103
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Authors
Aya El-Gamal1; Mohammed Kassab2; Ahmed Abd-Elmaksoud1; Amany Farag1; Hany Marie1
1Department of Cytology and Histology, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt
2Department of Cytology and Histology, Faculty of Veterinary Medicine, Kafrelshiekh University, Kafrelshiek 33511, Egypt
Abstract
The field of stem cells has attracted the attention of many researchers as a hope to treat many incurable diseases because of their ability of self-renewal and differentiation into a specific type of cells that compensates damaged cells. Many studies have been published to confirm their effectiveness but there are some obstacles that limit their clinical applications. One of these obstacles is xenogenic fetal bovine serum (FBS); the main proliferative source for stem cell culture with subsequent risk of infection transmission or immunogenic problems. So, in our study, we aimed to replace the xenogenic FBS with xeno-free blood derivative. We tested the effect of different concentrations of activated pure platelet rich plasma (P-PRP); one of the blood derivatives on proliferation of adipose derived mesenchymal stem cells (AD-MSCs) in comparison to FBS and found that 20% activated P-PRP followed by 10% activated P-PRP increased the proliferation rate of AD-MSCs more than 10% FBS. KEYWORDS AD-MSCs, P-PRP, FBS.
Keywords
AD-MSCs; P-PRP; FBS
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