EFFECT OF IN VITRO PRESERVATION ON THE RAPD PROFILES IN "KORONAIKI" AND "KALAMATA" OLIVE CULTIVARS (OLEA europaea L.)
Saeed, W., Hassan, H., Gazy, S., Shaban, E. (2005). EFFECT OF IN VITRO PRESERVATION ON THE RAPD PROFILES IN "KORONAIKI" AND "KALAMATA" OLIVE CULTIVARS (OLEA europaea L.). EKB Journal Management System, 13(2), 441-452. doi: 10.21608/ajs.2005.15493
Wafaa T Saeed; H Z Hassan; Saffia M.M Gazy; Eglal M.S Shaban. "EFFECT OF IN VITRO PRESERVATION ON THE RAPD PROFILES IN "KORONAIKI" AND "KALAMATA" OLIVE CULTIVARS (OLEA europaea L.)". EKB Journal Management System, 13, 2, 2005, 441-452. doi: 10.21608/ajs.2005.15493
Saeed, W., Hassan, H., Gazy, S., Shaban, E. (2005). 'EFFECT OF IN VITRO PRESERVATION ON THE RAPD PROFILES IN "KORONAIKI" AND "KALAMATA" OLIVE CULTIVARS (OLEA europaea L.)', EKB Journal Management System, 13(2), pp. 441-452. doi: 10.21608/ajs.2005.15493
Saeed, W., Hassan, H., Gazy, S., Shaban, E. EFFECT OF IN VITRO PRESERVATION ON THE RAPD PROFILES IN "KORONAIKI" AND "KALAMATA" OLIVE CULTIVARS (OLEA europaea L.). EKB Journal Management System, 2005; 13(2): 441-452. doi: 10.21608/ajs.2005.15493

EFFECT OF IN VITRO PRESERVATION ON THE RAPD PROFILES IN "KORONAIKI" AND "KALAMATA" OLIVE CULTIVARS (OLEA europaea L.)

Arab Universities Journal of Agricultural Sciences
Article 18, Volume 13, Issue 2, September 2005, Page 441-452  XML PDF (538.42 K)
Document Type: Original Article
DOI: 10.21608/ajs.2005.15493
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Authors
Wafaa T Saeed* 1; H Z Hassan2; Saffia M.M Gazy3; Eglal M.S Shaban1
1Hort. Res. Inst., Agric. Res. Cent., Min. Agric., Cairo, Egypt
2Dept. Bot., Fac. Sci., Ain Shams University, Cairo, Egypt
3Dept. Bot., Fac. Sci., Helwan University, Cairo, Egypt
Abstract
The present work aimed to establish a protocol to minimize the potential effect of in-vitro preservation for 8 months of the two olive cultivars i.e Koronaiki and Kalamata. Three culture conditions (treatments) were chosen to investigate the most suitable one for minimizing somaclonal variations after the process of in vitro preservation. The conditions include MS medium plus mannitol, MS medium plus low temperature and MS medium plus naphthaline acetic acid. RAPD-based finger-printing was employed to determine the effects of the different chemical constituents and other culture conditions on the extent of somaclonal variations after 8 months of in vitro preservation at the DNA level. Four arbitrary primers (OPA-04, -07, -14 and -16) were successfully utilized to achieve such goal. The obtained results showed that Mannitol has more pronounced effect than the other investigated preservation culture and conditions for decreasing DNA alterations.
Keywords
olive; Koronaiki; Kalamata; Preservation; DNA; primers
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