IN VITRO PROPAGATION OF THREE ALMOND CULTIVARS AND THE ALMOND – PEACH HYBRID ROOT-STOCK“HANSEN”
El-Hammady, A., Wanas, W., Abd El-Hamid, A., El-Salem, M. (2005). IN VITRO PROPAGATION OF THREE ALMOND CULTIVARS AND THE ALMOND – PEACH HYBRID ROOT-STOCK“HANSEN”. EKB Journal Management System, 13(2), 481-499. doi: 10.21608/ajs.2005.15512
A El-Hammady; Wafaa H Wanas; A Abd El-Hamid; M J El-Salem. "IN VITRO PROPAGATION OF THREE ALMOND CULTIVARS AND THE ALMOND – PEACH HYBRID ROOT-STOCK“HANSEN”". EKB Journal Management System, 13, 2, 2005, 481-499. doi: 10.21608/ajs.2005.15512
El-Hammady, A., Wanas, W., Abd El-Hamid, A., El-Salem, M. (2005). 'IN VITRO PROPAGATION OF THREE ALMOND CULTIVARS AND THE ALMOND – PEACH HYBRID ROOT-STOCK“HANSEN”', EKB Journal Management System, 13(2), pp. 481-499. doi: 10.21608/ajs.2005.15512
El-Hammady, A., Wanas, W., Abd El-Hamid, A., El-Salem, M. IN VITRO PROPAGATION OF THREE ALMOND CULTIVARS AND THE ALMOND – PEACH HYBRID ROOT-STOCK“HANSEN”. EKB Journal Management System, 2005; 13(2): 481-499. doi: 10.21608/ajs.2005.15512

IN VITRO PROPAGATION OF THREE ALMOND CULTIVARS AND THE ALMOND – PEACH HYBRID ROOT-STOCK“HANSEN”

Arab Universities Journal of Agricultural Sciences
Article 22, Volume 13, Issue 2, September 2005, Page 481-499  XML PDF (544.22 K)
Document Type: Original Article
DOI: 10.21608/ajs.2005.15512
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Authors
A El-Hammady* 1; Wafaa H Wanas1; A Abd El-Hamid2; M J El-Salem1
1Department of Horticulture, Faculty of Agriculture, Ain Shams University, Shoubra El-Kheima, Cairo, Egypt
2Department of Horticulture, Faculty of Agriculture, Ain Shams University, Shoubra El-Kheima, Cairo, Egypt.
Abstract
Stem node explants of three almond (Prunus dulcis Mill) cultivars (Om – Elfahm, M-Dalt and Ne Plus Ultra) and the hybrid rootstock “Hansen” cv. were suc-cessfully established and proliferated on Murashige and Skoog medium (MS) sup-plemented with benzyl adenine (BA) at 0.0 - 2.0 mgl -1 and indol butyric acid (IBA) at 0.0 - 0.1 mgl-1 .The highest bud development percentage of stem node explant was obtained with medium contained BA at 2 mgl-1 with or without IBA at 0.1 mgl-1 for M – Dalet and Ne Plus Ultra almond cultivars compared with other treatments. The longest shoot during establishment stage was recorded by “Hansen” rootstock cultured in MS with BA at 2.0 mgl-1 plus IBA at 0.01 mgl-1.Using BA at 1.0 mgl-1 plus IBA at 0.1 mgl-1 and BA at 2.0 mgl -1 plus 0.05 or 0.1 mgl -1gave the highest number of proliferated shoots for Om – Elfahm, M-Dalt and Ne Puls Ultra cvs. and “Hansen”, respectively in the 3rd subculture. The best average proliferated shoot length was achieved with medium supplemented with BA at 1.0 mgl-1 plus IBA at 0.05 mgl-1 or 0.1 mgl-1 for M-Dalt, Ne Puls Ultra and “Hansen” respectively, where-as, using BA at 2.0 mgl -1 plus IBA at 0.1 mgl -1 recorded the longest average prolif-erated shoot for Om- Elfahm cultivar. Microshoots were rooted in modified Bourgin and Nitsch medium supplemented with IBA or NAA at 1.0 mgl-1 only or combination (0.2 IBA mgl-1 + NAA 0.4 mgl-1, IBA 0.4 mgl-1 + NAA 0.8 mgl-1 and IBA 0.5 mgl-1 + NAA 1.0 mgl-1). The large callus (more than 10 mm diameter) was recorded with “Hansen” rootstock and Om – Elfahm treated by IBA at 0.4 mgl -1 plus NAA at 0.8 mgl -1. The greatest rooting percentage (66.67%) was obtained with medium contained IBA at 0.2 or 0.4 mgl-1 plus NAA at 0.4 or 0.8 mgl-1 for “Hansen” rootstock and Ne Plus Ultra shoots.
Keywords
propagation; Almond cvs; BA; IBA; multiplication; subculture; Rooting
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