Experimental Rat Model of Diabetic Nephropathy: RAGE Detection and Effect of Spironolactone Monotherapy
Nabil, M., Raafat, M., Waked, E., Badawy, A., Salah, M. (2015). Experimental Rat Model of Diabetic Nephropathy: RAGE Detection and Effect of Spironolactone Monotherapy. EKB Journal Management System, 58(1), 120-128. doi: 10.12816/0009366
Malak Nabil; Manar Raafat; Emam Waked; Afkar Badawy; Mohamed Salah. "Experimental Rat Model of Diabetic Nephropathy: RAGE Detection and Effect of Spironolactone Monotherapy". EKB Journal Management System, 58, 1, 2015, 120-128. doi: 10.12816/0009366
Nabil, M., Raafat, M., Waked, E., Badawy, A., Salah, M. (2015). 'Experimental Rat Model of Diabetic Nephropathy: RAGE Detection and Effect of Spironolactone Monotherapy', EKB Journal Management System, 58(1), pp. 120-128. doi: 10.12816/0009366
Nabil, M., Raafat, M., Waked, E., Badawy, A., Salah, M. Experimental Rat Model of Diabetic Nephropathy: RAGE Detection and Effect of Spironolactone Monotherapy. EKB Journal Management System, 2015; 58(1): 120-128. doi: 10.12816/0009366

Experimental Rat Model of Diabetic Nephropathy: RAGE Detection and Effect of Spironolactone Monotherapy

The Egyptian Journal of Hospital Medicine
Article 13, Volume 58, Issue 1, January 2015, Page 120-128  XML PDF (653.79 K)
Document Type: Original Article
DOI: 10.12816/0009366
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Authors
Malak Nabil1; Manar Raafat1; Emam Waked1; Afkar Badawy2; Mohamed Salah3
1Nephrology Department Theodor Bilharz Research Institute, Imbaba, Giza.
2Pathology Department Theodor Bilharz Research Institute, Imbaba, Giza.
3Veterinary Department Theodor Bilharz Research Institute, Imbaba, Giza.
Abstract
Background: The glycation process results in formation of advanced glycation end products (AGEs), which accumulate in different organs at an accelerated rate in diabetes, resulting in alteration of both structure and function. This effect is via the receptor for AGES (RAGE), which is a signaling receptor leading to profibrotic reactions. The renin angiotensin aldosterone system (RAAS) is activated in diabetic nephropathy (DN) and leads to more renal damage. This is inhibited by angiotensin converting enzyme inhibitors (ACEIs) and angiotensin receptor blockers (ARBs) and mineralocorticoid receptor blockers (MRBs).Aim of the study: to show the monotherapeutic effect of spironolactone in diabetic nephropathy and to detect RAGE. Method: Diabetes was induced in rats by streptozotocin. Three weeks after,spironolactone (SPL) was given for 4 weeks. Then, control, diabetic and treated rats were sacrificed. Results: The results of blood chemistry at the end of 4 weeks showed statistical increase in serum sodium, potassium and urea with no effect on serum creatinine or blood glucose. Kidney pathological injuries were attenuated by SPL also, RAGE deposition compared to the diabetics. The study showed RAGE deposition in the experimental DN and confirmed the beneficial effects of MRB in DN.
 
Keywords
diabetic nephropathy; receptor for AGES (RAGE); the renin angiotensin aldosterone system(RAAS); spironolactone(SPL)
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