Physiological and Molecular Defense Level in Potato Cultivars against Potato Virus X | ||||
| Annals of Agricultural Science, Moshtohor | ||||
| Article 20, Volume 58, Issue 4, December 2020, Page 1079-1088 PDF (583.44 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/assjm.2020.155407 | ||||
 View on SCiNiTO | ||||
| Author | ||||
| Noha K. El-Dougdoug* | ||||
| Microbiology and Botany Dept. Faculty of Science, Benha University, Egypt. | ||||
| Abstract | ||||
| Potato virus X (PVX) causes severe losses in worldwide crops of family Solanaceae. Dramatic biochemical changes in virus infected plants due to decrease in qualitative and quantitative of yield crops. The current study demonstrate the changes of biochemical responses as a defense level and resistance genes in infected plants against virus infection. PVX isolate was confirmed by double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) . This study was performed on five cultivars of imported seeds potato PVX tested, Diamond , Hermes , Lady Rosetta , Nicola , and Spunta . All potato cultivars were infected with PVX isolate. The five potato cultivars showed variations symptoms development, disease severity and virus concentration .The potato cultivars against PVX infection exhibited significantly increase in physiological alterations (electrolyte leakage, cell membrane stability and transpiration rate) and biochemical responses defense (total proline, stabilizing phenols, scavenging enzymes, catalase, peroxidase, polyphenol oxidase, superoxide dismutase, activities) and RX1 , RX2 , RX3 genes resistance compared to healthy ones. According to viral assessment, physiological parameters, biochemical responses and RX resistance genes , the five potato cultivars were divided to resistant cv., Diamond, tolerant cv., Hermes, Lady Rosetta, Nicola and susceptible cv., Spunta | ||||
| Keywords | ||||
| Potato cultivars; PVX; scavenging enzymes activities and physiological marker response | ||||
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