Cytotoxicity of Silver Nanoparticles in Mice Liver Cells: An Ultrastructure Study
El-Habit, O., Mousa, E., Hassan, B. (2014). Cytotoxicity of Silver Nanoparticles in Mice Liver Cells: An Ultrastructure Study. EKB Journal Management System, 57(1), 554-564.
Ola H. El-Habit; Eman A. Mousa; Basma N. Hassan. "Cytotoxicity of Silver Nanoparticles in Mice Liver Cells: An Ultrastructure Study". EKB Journal Management System, 57, 1, 2014, 554-564.
El-Habit, O., Mousa, E., Hassan, B. (2014). 'Cytotoxicity of Silver Nanoparticles in Mice Liver Cells: An Ultrastructure Study', EKB Journal Management System, 57(1), pp. 554-564.
El-Habit, O., Mousa, E., Hassan, B. Cytotoxicity of Silver Nanoparticles in Mice Liver Cells: An Ultrastructure Study. EKB Journal Management System, 2014; 57(1): 554-564.

Cytotoxicity of Silver Nanoparticles in Mice Liver Cells: An Ultrastructure Study

The Egyptian Journal of Hospital Medicine
Article 17, Volume 57, Issue 1, October 2014, Page 554-564  XML PDF (1.97 MB)
Document Type: Original Article
View on SCiNiTO View on SCiNiTO
Authors
Ola H. El-Habit1; Eman A. Mousa2; Basma N. Hassan1
1Faculty of Science, Zoology and Entomology Department, Helwan University, Egypt
2Faculty of Science, Zoology Department, Kafr El-sheikh University, Egypt
Abstract
   Introduction: Nanoparticles of silver have many important applications and are among the most commonly used nanomaterials. They are increasingly used in a variety of both medical and consumer products which includes: spectrally selective coating for solar energy absorption and intercalation material for electrical batteries, as optical receptors, polarizing filters, catalysts in chemical reaction and bio-labeling. Nanosilver (Ag-NP) has both antibacterial and antiviral activity. Yet, the knowledge about the systemic toxicity of nanosilver is relatively limited.
The aim of work: To evaluate the potential toxicity of small size 10nm silver nanoparticles using two different doses (0.1 ml and 0.4 ml) focusing on the ultrastructural changes occurring in mice hepatocytes.
    The methods: This study was performed using three groups of mice. The animals of the first group were given a daily intravenous injection of 0.1 ml of silver nanoparticles for 28 consecutive days. The second group was treated with 0.4 ml of silver nanoparticles for 28 consecutive days. The third group served as a control group in which the animals did not receive any vehicle. The study was focused on the ultrastructure of the liver.
    The results: Ultrastructure observations of liver cells of miceTreated with any of the two doses (0.1 and 0.4 ml) of 10 nm Ag-NP indicated severe accumulation of dark deposits of Ag-NP in the cytoplasm and the cell organelles.
    Conclusion: Our study revealed that nanosilver used in doses of 0.1 and 0.4 ml led to deposits in the cells and induced damage of cell components especially the nucleus, mitochondria and chromatin.
 
 
Keywords
nanosilver; Cytotoxicity; Liver; mitochondria
Statistics
Article View: 113
PDF Download: 328