Molecular and Physiological Detection of Cyst Nematodes on Potato During Plant Nematode Interactions
Elkobrosy, D., Aseel, D., Abdelsalam, N., El-Saedy, M., Shama, S., Hafez, E. (2018). Molecular and Physiological Detection of Cyst Nematodes on Potato During Plant Nematode Interactions. EKB Journal Management System, 39(July-September), 478-481. doi: 10.21608/asejaiqjsae.2018.15649
Dina H. Elkobrosy; Dalia G. Aseel; Nader R. Abdelsalam; Mohamed A. El-Saedy; Saad Shama; Elsayed E. Hafez. "Molecular and Physiological Detection of Cyst Nematodes on Potato During Plant Nematode Interactions". EKB Journal Management System, 39, July-September, 2018, 478-481. doi: 10.21608/asejaiqjsae.2018.15649
Elkobrosy, D., Aseel, D., Abdelsalam, N., El-Saedy, M., Shama, S., Hafez, E. (2018). 'Molecular and Physiological Detection of Cyst Nematodes on Potato During Plant Nematode Interactions', EKB Journal Management System, 39(July-September), pp. 478-481. doi: 10.21608/asejaiqjsae.2018.15649
Elkobrosy, D., Aseel, D., Abdelsalam, N., El-Saedy, M., Shama, S., Hafez, E. Molecular and Physiological Detection of Cyst Nematodes on Potato During Plant Nematode Interactions. EKB Journal Management System, 2018; 39(July-September): 478-481. doi: 10.21608/asejaiqjsae.2018.15649

Molecular and Physiological Detection of Cyst Nematodes on Potato During Plant Nematode Interactions

Alexandria Science Exchange Journal
Article 12, Volume 39, July-September - Serial Number 3, July 2018, Page 478-481  XML PDF (201.46 K)
Document Type: Original Article
DOI: 10.21608/asejaiqjsae.2018.15649
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Authors
Dina H. Elkobrosy* 1; Dalia G. Aseel* 1; Nader R. Abdelsalam* 2; Mohamed A. El-Saedy* 3; Saad Shama* 4; Elsayed E. Hafez* 5
1Plant Protection and Biomolecular Diagnosis Department, Arid Lands Cultivation Research Institute, City of Scientific Research and Technological Applications, New Borg El-Arab City, 21934 Alexandria, Egypt.
2Agricultural Botany Department, Faculty of Agriculture (Saba Basha), Alexandria University, 21531 Alexandria,Egypt.
3Plant Pathology Department, Faculty of Agriculture (Elshatby),Alexandria University, 21545 Alexandria, Egypt.
4Agricultural Botany Department, Faculty of Agriculture (Saba Basha), Alexandria University, 21531 Alexandria, Egypt.
5Plant Protection and Biomolecular Diagnosis Department, Arid Lands Cultivation Research Institute, City of Scientific Research and Technological Applications, New Borg El-Arab City, 21934 Alexandria, Egypt.
Abstract
Cyst nematodes involve over than 90 known species into six genera, G. rostochiensis and G. pallida are most important pathogens of potato crops where their economically a yield losses. The aim of present investigation is characterization the cyst nematode species (G. rostochiensis) using molecular techniques during plant-nematode interaction. The molecular identification of cyst nematode was carried out using ITS PCR. The results of ITS-PCR showed two bands with different molecular sizes 238 bp and 370 bp. The metabolic activation of potato leaves infected with cyst nematodes was detected. The results confirmed that, G.rostochiensis significantly elevated the contents of shoots lipid peroxidation (MDA) as compared to the untreated healthy plants.
 
Keywords
Cyst nematodes; ITS PCR; potato
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